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Gewählte Publikation:

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Publikationstyp: Zeitschriftenaufsatz
Dokumenttyp: Originalarbeit

Jahr: 2013

AutorInnen: Kumar, G; Abd-Elfattah, A; Soliman, H; El-Matbouli, M

Titel: Establishment of medium for laboratory cultivation and maintenance of Fredericella sultana for in vivo experiments with Tetracapsuloides bryosalmonae (Myxozoa).

Quelle: J Fish Dis. 2013; 36(2):81-88



Autor/innen der Vetmeduni Vienna:

Abd-Elfattah Ahmed
El-Matbouli Mansour
Kumar Gokhlesh
Soliman Hatem

Beteiligte Vetmed-Organisationseinheiten
Universitätsklinik für Geflügel und Fische, Klinische Abteilung für Fischmedizin


Zugehörige(s) Projekt(e): Genexpression und -funktion von Tetracapsuloide bryosalmonae


Abstract:
The freshwater bryozoan Fredericella sultana (Blumenbach) is the most common invertebrate host of the myxozoan parasite Tetracapsuloides bryosalmonae, the causative agent of proliferative kidney disease in salmonid fish. Culture media play an important role in hatching of statoblasts and maintaining clean bryozoan colonies for Malacosporea research. We developed a novel culture medium, Bryozoan Medium C (BMC), for the cultivation and maintenance of F. sultana under laboratory conditions. Statoblasts of F. sultana were successfully hatched to produce transparent-walled, specific pathogen-free (SPF) colonies that were maintained >12 months in BMC at pH 6.65. Tetracapsuloides bryosalmonae was successfully transmitted from infected brown trout, Salmo trutta L., to newly hatched F. sultana colonies in BMC, then from the infected bryozoan to SPF brown trout. This study demonstrated the utility of BMC (pH 6.65) for hatching statoblasts, long-term cultivation of clean and transparent bryozoan colonies and maintenance of the Tetracapsuloides bryosalmonae life cycle in the laboratory for molecular genetic research and other studies such as host-parasiteinteraction.

Keywords Pubmed: Animals
Bryozoa/growth & development*
Bryozoa/parasitology*
Culture Techniques
Fish Diseases/parasitology*
Fish Diseases/transmission
Food Chain
Myxozoa/physiology*
Parasitic Diseases, Animal/parasitology*
Parasitic Diseases, Animal/transmission
Parasitology/methods*
Trout


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