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Gewählte Publikation:

Publikationstyp: Zeitschriftenaufsatz
Dokumentart: Originalarbeit

Publikationsjahr: 2013

AutorInnen: Talker, SC; Käser, T; Reutner, K; Sedlak, C; Mair, KH; Koinig, H; Graage, R; Viehmann, M; Klingler, E; Ladinig, A; Ritzmann, M; Saalmüller, A; Gerner, W

Titel: Phenotypic maturation of porcine NK- and T-cell subsets.

Quelle: Dev Comp Immunol. 2013; 40(1):51-68

Autor/innen der Vetmeduni Vienna:

Gerner Wilhelm
Graage Robert
Käser Tobias
Klingler Eva
Koinig Hanna
Ladinig Andrea
Mair Kerstin
Reutner Katharina
Ritzmann Mathias
Saalmüller Armin
Sedlak Corinna
Viehmann Miriam

Beteiligte Vetmed-Organisationseinheiten
Institut für Immunologie
Universitätsklinik für Schweine

Detailed information concerning the development of the immune system in young pigs is still rudimental. In the present study, we analyzed changes in phenotype and absolute numbers of natural killer cells, γδ T cells, T helper cells, regulatory T cells and cytolytic T cells in the blood of pigs from birth to six months of age. For each lymphocyte subpopulation, a combination of lineage and differentiation markers was investigated by six-color flow cytometry. Major findings were: (i) absolute numbers of γδ T cells strongly increased from birth until 19-25 weeks of age, indicating an important role for these cells during adolescence; (ii) phenotype of T helper cells changed over time from CD8α(-)SLA-DR(-)CD27(+) towards CD8α(+)SLA-DR(+)CD27(-) but CD45RC(-) T helper cells were found immediately after birth, therefore questioning the role of this marker for the identification of T-helper memory cells; (iii) for cytolytic T cells, putative phenotypes for early effector (CD3(+)CD8αβ(+)perforin(+)CD27(dim)) and late effector or memory cells (CD3(+)CD8αβ(+)perforin(+)CD27(-)) could be identified.

Keywords Pubmed: Animals
Animals, Newborn
Cell Differentiation
Immunologic Memory
Killer Cells, Natural/immunology*
Receptors, Antigen, T-Cell, gamma-delta/metabolism
T-Lymphocyte Subsets/immunology*
T-Lymphocytes, Cytotoxic/immunology*
T-Lymphocytes, Helper-Inducer/immunology*
T-Lymphocytes, Regulatory/immunology*

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