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Gewählte Publikation:

Publikationstyp: Zeitschriftenaufsatz
Dokumenttyp: Originalarbeit

Jahr: 2010

AutorInnen: Kumar, G; Rathore, G; Sengupta, U; Kapoor, D; Lakra, WS

Titel: Production of monoclonal antibodies specific to major outer membrane protein of Edwardsiella tarda.

Quelle: Comp Immunol Microbiol Infect Dis. 2010; 33(2):133-144

Autor/innen der Vetmeduni Vienna:

Kumar Gokhlesh

Diese Publikation wurde nicht im Namen der Vetmeduni Vienna erstellt und ist deshalb ausschließlich der persönlichen Publikationsliste des/der Autors/Autorin zugeordnet!

Edwardsiella tarda is an important cause for hemorrhagic septicemia in fish and gastro and extra-intestinal infections in humans. Monoclonal antibodies (MAbs) were produced against outer membrane proteins (OMPs) of E. tarda ET-7, isolated from diseased snakehead (Ophiocephalus punctatus). Two stable hybridoma clones, designated as 3F10 and 2C3 MAbs were found to be potentially specific for E. tarda by indirect enzyme linked immunosorbent assay (ELISA). These MAbs recognized major immunogenic OMP band at 44kDa in Western blotting. Both MAbs belonged to the IgG1 isotype and recognized different epitopes of OMP as seen by competitive ELISA. These MAbs strongly reacted with all 17 isolates of E. tarda used in our study by indirect ELISA and Western blotting. Interestingly, no reaction was observed with the reference strain of E. tarda (MTCC 2400). The sensitivity of 3F10 MAb to detect whole cells of E. tarda was up to a level of 1x10(4)CFU/ml in indirect ELISA. No cross-reactivity of MAbs were seen with Escherichia coli, Salmonella arizonae, Pseudomonas fluorescens, Aeromonas hydrophila, Vibrio cholerae, Flavobacterium ferrugineum and Mycobacterium tuberculosis. These MAbs could be used for specific detection of E. tarda infection in fish by immunoassays.

Keywords Pubmed: Animals
Antibodies, Bacterial/biosynthesis*
Antibodies, Bacterial/immunology
Antibodies, Monoclonal/biosynthesis*
Antibodies, Monoclonal/immunology
Bacterial Outer Membrane Proteins/chemistry
Bacterial Outer Membrane Proteins/immunology*
Bacterial Outer Membrane Proteins/isolation & purification
Blotting, Western
Cross Reactions
Edwardsiella tarda/immunology*
Edwardsiella tarda/isolation & purification
Enterobacteriaceae Infections/diagnosis
Enterobacteriaceae Infections/immunology
Enterobacteriaceae Infections/microbiology
Enterobacteriaceae Infections/veterinary*
Enzyme-Linked Immunosorbent Assay
Fish Diseases/diagnosis*
Fish Diseases/immunology
Fish Diseases/microbiology
Immunoglobulin G/biosynthesis
Immunoglobulin G/immunology
Sensitivity and Specificity

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