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Gewählte Publikation:

Publikationstyp: Zeitschriftenaufsatz
Dokumenttyp: Originalarbeit

Jahr: 2005

AutorInnen: Roth-Walter, F; Schöll, I; Untersmayr, E; Ellinger, A; Boltz-Nitulescu, G; Scheiner, O; Gabor, F; Jensen-Jarolim, E

Titel: Mucosal targeting of allergen-loaded microspheres by Aleuria aurantia lectin.

Quelle: Vaccine. 2005; 23(21):2703-2710



Autor/innen der Vetmeduni Vienna:

Jensen-Jarolim Erika
Roth-Walter Franziska

Diese Publikation wurde nicht im Namen der Vetmeduni Vienna erstellt und ist deshalb ausschließlich der persönlichen Publikationsliste des/der Autors/Autorin zugeordnet!


Abstract:
Murine intestinal M-cells express alpha-L-fucose residues. We constructed alpha-L-fucose-targeting particles for oral immunotherapy of IgE-mediated allergy. Poly(D,L-lactic-co-glycolic acid)-microspheres were loaded with birch pollen allergens, and functionalised with the alpha-L-fucose specific Aleuria aurantia lectin (AAL). The AAL-microspheres had a size of 1-3 microm, protected the entrapped allergens from gastric degradation and released 46.6+/-1.3% allergen over 21 days in vitro. Oral gavages of AAL-particles to naive BALB/c mice induced birch pollen-specific IgG2a, but not IgG1 antibodies. We conclude that targeting allergens to alpha-L-fucose-receptor bearing cells using AAL-microspheres induces specific Th1-antibody responses possibly counteracting Th2-dominated allergy, and therefore provides a potentially useful formulation for oral immunotherapy.

Keywords Pubmed: Allergens/administration & dosage*
Animals
Betula/immunology
Caco-2 Cells
Desensitization, Immunologic*
Enterocytes/metabolism
Female
Humans
Immunoglobulin G/blood
Lactic Acid/administration & dosage*
Lectins/administration & dosage
Lectins/metabolism*
Mice
Mice, Inbred BALB C
Microspheres*
Pollen/immunology*
Polyglycolic Acid/administration & dosage*
Polymers/administration & dosage*


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