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Gewählte Publikation:

Publikationstyp: Zeitschriftenaufsatz
Dokumenttyp: Originalarbeit

Jahr: 2005

AutorInnen: Roth-Walter, F; Bohle, B; Schöll, I; Untersmayr, E; Scheiner, O; Boltz-Nitulescu, G; Gabor, F; Brayden, DJ; Jensen-Jarolim, E

Titel: Targeting antigens to murine and human M-cells with Aleuria aurantia lectin-functionalized microparticles.

Quelle: Immunol Lett. 2005; 100(2):182-188



Autor/innen der Vetmeduni Vienna:

Jensen-Jarolim Erika
Roth-Walter Franziska

Diese Publikation wurde nicht im Namen der Vetmeduni Vienna erstellt und ist deshalb ausschließlich der persönlichen Publikationsliste des/der Autors/Autorin zugeordnet!


Abstract:
Neuraminidases act as a virulence factors for several pathogens that invade the human body through Peyer"s patch M-cells. Because of the structural similarity of Aleuria aurantia lectin (AAL) to neuraminidases, we hypothesized that AAL might also target human M-cells. In an in vitro human M-cell co-culture model significantly more particles were transported across the epithelium when microparticles were functionalized with AAL versus those that were not. Moreover, high concentrations of AAL induced no detectable cytotoxic effects on the related intestinal epithelial cell cultures, epithelial Caco2- and HT29-MTX-E12-cells. Upon incubation with AAL, PBMCs of allergic volunteers proliferated in response to AAL and secreted the cytokines, IL-2, IFN-gamma, IL-10 and IL-5 in a concentration-dependent manner, indicating immune-stimulatory properties of the lectin. We conclude that AAL-coated microparticles may have the potential to target entrapped antigens to human M-cells for oral vaccination.

Keywords Pubmed: Allergens/immunology
Caco-2 Cells
Coculture Techniques
Dose-Response Relationship, Immunologic
Drug Delivery Systems*
HT29 Cells
Humans
Interferon-gamma/biosynthesis
Interleukin-2/biosynthesis
Lectins*/immunology
Microspheres*
Monocytes/cytology
Monocytes/immunology*
Peyer"s Patches/immunology*
Pollen/immunology
Vaccines/administration & dosage*


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