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Gewählte Publikation:

Publikationstyp: Zeitschriftenaufsatz
Dokumenttyp: Originalarbeit

Jahr: 2004

AutorInnen: Walter, F; Schöll, I; Untersmayr, E; Ellinger, A; Boltz-Nitulescu, G; Scheiner, O; Gabor, F; Jensen-Jarolim, E

Titel: Functionalisation of allergen-loaded microspheres with wheat germ agglutinin for targeting enterocytes.

Quelle: Biochem Biophys Res Commun. 2004; 315(2):281-287



Autor/innen der Vetmeduni Vienna:

Jensen-Jarolim Erika
Roth-Walter Franziska

Diese Publikation wurde nicht im Namen der Vetmeduni Vienna erstellt und ist deshalb ausschließlich der persönlichen Publikationsliste des/der Autors/Autorin zugeordnet!


Abstract:
In this study, we constructed particles applicable for oral immunotherapy of type I allergy by protecting allergens from digestion and supporting intestinal antigen uptake. Therefore, birch-pollen allergens were entrapped in poly(d,l-lactic-co-glycolic acid) microspheres by spray-drying rendering microspheres with a main population of 1-3microm. Microspheres were further coated with wheat germ agglutinin (WGA) to target enterocytes. Coating with WGA did not alter the surface characteristics of the microspheres as demonstrated in scanning electron microscopy. Binding of WGA was specific and could be inhibited by chitotriose to 14.7+/-6.9%. Comparable amounts of allergen were released from both particle-types with 46.3+/-1.7% and 44.5+/-2.6% during 21 days. Simulating gastric digestion in vitro, antigenicity of allergens entrapped in WGA-microspheres was preserved to 59.8+/-1.5% even after 2h. Feedings of BALB/c mice with WGA-microspheres induced higher levels of allergen-specific IgG-levels than gavages of uncoated microparticles or naked protein. Thus, we conclude that WGA-microspheres are suitable vehicles for oral delivery and mucosal targeting due to lectin-mediated bioadhesion.

Keywords Pubmed: Allergens/chemistry*
Animals
Betula
Biocompatible Materials
Caco-2 Cells
Carbohydrates/chemistry
Cell Line
Cells, Cultured
Dose-Response Relationship, Drug
Enterocytes/metabolism*
Enzyme-Linked Immunosorbent Assay
Female
Fluorescein/pharmacology
Humans
Immunoglobulin G/chemistry
Immunotherapy
Lactic Acid/chemistry
Lectins/chemistry
Mice
Mice, Inbred BALB C
Microscopy, Electron, Scanning
Microspheres*
Plant Lectins/chemistry
Polyglycolic Acid/chemistry
Polymers/chemistry
Protein Binding
Time Factors
Trisaccharides/metabolism
Wheat Germ Agglutinins/chemistry*


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