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Gewählte Publikation:

Publikationstyp: Zeitschriftenaufsatz
Dokumenttyp: Originalarbeit

Jahr: 2014

AutorInnen: Kumar, G; Saleh, M; Abdel-Baki, AA; Al-Quraishy, S; El-Matbouli, M

Titel: In vitro cultivation model for Heterosporis saurida (Microsporidia) isolated from lizardfish, Saurida undosquamis (Richardson).

Quelle: J Fish Dis. 2014; 37(5):443-449



Autor/innen der Vetmeduni Vienna:

El-Matbouli Mansour
Kumar Gokhlesh
Saleh Mona

Beteiligte Vetmed-Organisationseinheiten
Universitätsklinik für Geflügel und Fische, Klinische Abteilung für Fischmedizin


Abstract:
Heterosporis saurida is a microsporidian that infects lizardfish, Saurida undosquamis (Richardson, 1848), in the Arabian Sea. Spores were isolated from infected lizardfish and used to infect derived fish cell lines: common carp brain (CCB), epithelioma papulosum cyprinid (EPC), fathead minnow epithelial (FHM), rainbow trout gonad (RTG), bluegill fry (BF-2) and chinook salmon embryo (CHSE). Non-fish cell lines were also tested that include: insect (SF-9), rabbit (RK-13) and African green monkey (Vero E6). No growth of H. saurida was observed in any fish cell line, SF-9 or Vero E6 cell lines. H. saurida spores grew only in RK-13 cell line and were detected by immunofluorescence. Developmental stages of H. saurida were seen in RK-13 cells by light and transmission electron microscopy, and species identification was confirmed by sequencing. This study demonstrated that H. saurida was able to proliferate in the mammalian RK-13 cell line, which thus represents an in vitro model for conducting molecular genetics and cell-pathogen interaction studies of Heterosporis.

Keywords Pubmed: Animals
Cell Culture Techniques/veterinary
Fish Diseases/parasitology*
Microsporidia/genetics
Microsporidia/isolation & purification*
Microsporidiosis/parasitology
Microsporidiosis/veterinary*
Polymerase Chain Reaction/veterinary


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