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Gewählte Publikation:

Publikationstyp: Zeitschriftenaufsatz
Dokumenttyp: Originalarbeit

Jahr: 2014

AutorInnen: Palme, N; Becher, AC; Merkl, M; Glösmann, M; Aurich, C; Schäfer-Somi, S

Titel: Immunolocalization of the cholesterol transporters ABCA1 and ABCG1 in canine reproductive tract tissues and spermatozoa.

Quelle: Reprod Domest Anim. 2014; 49(3):441-447



Autor/innen der Vetmeduni Vienna:

Aurich Christine
Becher-Deichsel Anja
Glösmann Martin
Merkl Maximiliane
Schäfer-Somi Sabine

Beteiligte Vetmed-Organisationseinheiten
Universitätsklinik für Kleintiere, Klinische Abteilung für Geburtshilfe, Gynäkologie und Andrologie
Plattform Besamung und Embryotransfer
VetCore


Abstract:
The mammalian sperm membrane undergoes cholesterol efflux during maturation and fertilization. Although ATP-binding cassette (ABC) transporters are known to transport cholesterol through cell membranes in other organs, their presence in canine testis, epididymis and sperm has not been proven to date. Hence, the aim of the present study was to localize the ABC transporters ABCA1 and ABCG1 in canine testicular and epididymidal tissue as well as in spermatozoa membranes. To this end, semen samples from 12 dogs as well as testicles and epididymides of four young and healthy dogs were prepared for immunohistochemistry, respectively. Capacitation and acrosome reaction (AR) were induced in aliquots of the semen samples before immunostaining to assess changes in the expression of ABCA1 and ABCG1. Evaluation by confocal microscopy revealed the presence of both ABCA1 and ABCG1 in canine testicles and of ABCA1 in the epididymides. In spermatozoa, only ABCA1 immunoreactivity was detected, mainly in the region of the acrosome and midpiece. After induction of capacitation, ABCA1 signal persisted in the acrosome but disappeared after AR, indicating a loss of ABCA1 with the loss of the acrosome. We conclude that ABCA1 and ABCG1 are expressed in canine testis, whereas only ABCA1 is expressed in epididymis and spermatozoa membrane, both transporters probably contributing to the regulation of membrane cholesterol content.

Keywords Pubmed: ATP Binding Cassette Transporter 1/analysis*
ATP-Binding Cassette Transporters/analysis*
Acrosome Reaction
Animals
Dogs/metabolism*
Epididymis/chemistry*
Immunohistochemistry
Male
Microscopy, Confocal
Sperm Capacitation
Spermatozoa/chemistry*
Testis/chemistry*


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