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Gewählte Publikation:

Publikationstyp: Zeitschriftenaufsatz
Dokumenttyp: Originalarbeit

Jahr: 2014

AutorInnen: Awad, WA; Ghareeb, K; Zentek, J

Titel: Mechanisms underlying the inhibitory effect of the feed contaminant deoxynivalenol on glucose absorption in broiler chickens.

Quelle: Vet J. 2014; 202(1):188-190



Autor/innen der Vetmeduni Vienna:

Awad Wageha
Ghareeb Khaled

Beteiligte Vetmed-Organisationseinheiten
Institut für Tierernährung und funktionelle Pflanzenstoffe
Universitätsklinik für Geflügel und Fische, Klinische Abteilung für Geflügelmedizin


Abstract:
Deoxynivalenol (DON), a major contaminant of cereals and grains, is of public health concern worldwide and has been shown to reduce the electrogenic transport of glucose. However, the full effects of Fusarium mycotoxins on nutrient absorption are still not clear. The aim of this study was to investigate whether decreased nutrient absorption was due to specific effects on transporter trafficking in the intestine and whether inhibition of phosphoinositol-3-kinase (PI-3-kinase) affected the electrogenic jejunal transport of glucose. Jejunal mucosa of 6-week-old broiler chickens were mounted in Ussing chambers and treated with DON, wortmannin (a specific inhibitor of PI-3-kinase), DON + wortmannin, phlorizin and cytochalasin B. DON was found to decrease the short-circuit current (Isc) after glucose addition. A similar decline in Isc after glucose addition was observed following pre-application of wortmannin, or phlorizin (Na(+)/glucose co-transporter, SGLT1 inhibitor). The results indicate that DON decreased glucose absorption in the absence of wortmannin or phlorizin but had no additional effect on glucose absorption in their presence. Glucose transport was not affected by cytochalasin B (facilitative glucose transporter, GLUT2 inhibitor). The study provides evidence that the suppressive effect of DON on the electrogenic transport of glucose may be due to an inhibitory activity of the PI3 kinase pathway and intestinal SGLT1. Furthermore, the effect of cytochalasin B on glucose transport in chicken tissues differs from that in mammals.

Keywords Pubmed: Androstadienes
Animal Feed/analysis*
Animals
Chickens/physiology*
Cytochalasin B
Diet/veterinary
Food Contamination
Glucose/metabolism
Glucose/pharmacokinetics*
Intestinal Mucosa/drug effects*
Intestinal Mucosa/physiology
Jejunum/drug effects*
Jejunum/physiology
Phlorhizin
Phosphatidylinositol 3-Kinases/antagonists & inhibitors
Phosphatidylinositol 3-Kinases/metabolism
Trichothecenes/toxicity*


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