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Gewählte Publikation:

Publikationstyp: Zeitschriftenaufsatz
Dokumenttyp: Originalarbeit

Jahr: 2014

AutorInnen: Mann, E; Wagner, M; Schmoll, F; Slaghuis, J; Schönenbrücher, H; Mester, P

Titel: Rapid testing and quantification of Salmonella in ileocaecal lymph nodes of Austrian pigs slaughtered for consumption.

Quelle: Res Vet Sci. 2014; 97(2):187-190



Autor/innen der Vetmeduni Vienna:

Mester Patrick-Julian
Selberherr Evelyne
Wagner Martin

Beteiligte Vetmed-Organisationseinheiten
Institut für Lebensmittelsicherheit, Lebensmitteltechnologie und öffentliches Gesundheitswesen in der Veterinärmedizin, Abteilung für Lebensmittelmikrobiologie


Zugehörige(s) Projekt(e): Präventive Veterinärmedizin


Abstract:
Traditionally, quantitative microbial risk assessment (QMRA) is based on culture-dependent technologies. However, molecular quantification could forge additional, detailed information. A prerequisite of quantitative real-time PCR in animal science is a tissue preparation method where large volumes of tissue material can be reduced and particularly target cells can be concentrated. An easy-to-use sample preparation method for food (Matrix-Lysis) was recently adapted to tissues and now permits quantification of target cells from up to 5 g of organic matrix. The aim of this study was to examine the suitability of Matrix-Lysis for quantification of Salmonella in porcine ileocaecal lymph nodes (ICLNs). After demonstrating constant recovery rates, ICLNs from 540 pigs were examined for Salmonella spp. with Matrix-Lysis. Samples were also analysed using ISO 6579:2002, a combined enrichment/qPCR method and a lateral flow test. It could be shown that qPCR coupled with Matrix-Lysis can contribute to QMRA in food safety by enabling reproducible quantitative data, even at low contamination rates.

Keywords Pubmed: Animals
Austria/epidemiology
DNA, Bacterial/genetics
Food Contamination/prevention & control
Food Microbiology/methods*
Hazard Analysis and Critical Control Points/methods
Ileocecal Valve/microbiology
Lymph Nodes/microbiology*
Real-Time Polymerase Chain Reaction/methods*
Real-Time Polymerase Chain Reaction/veterinary*
Risk Assessment/methods
Salmonella/genetics
Salmonella/isolation & purification*
Salmonella Infections, Animal/diagnosis
Salmonella Infections, Animal/epidemiology
Salmonella Infections, Animal/microbiology
Swine/microbiology*
Swine Diseases/diagnosis
Swine Diseases/epidemiology
Swine Diseases/microbiology


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