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Gewählte Publikation:

Publikationstyp: Zeitschriftenaufsatz
Dokumenttyp: Originalarbeit

Jahr: 2015

AutorInnen: Moti, N; Malcolm, T; Hamoudi, R; Mian, S; Garland, G; Hook, CE; Burke, GA; Wasik, MA; Merkel, O; Kenner, L; Laurenti, E; Dick, JE; Turner, SD

Titel: Anaplastic large cell lymphoma-propagating cells are detectable by side population analysis and possess an expression profile reflective of a primitive origin.

Quelle: Oncogene. 2015; 34(14):1843-1852



Autor/innen der Vetmeduni Vienna:

Kenner Lukas

Diese Publikation wurde nicht im Namen der Vetmeduni Vienna erstellt und ist deshalb ausschließlich der persönlichen Publikationsliste des/der Autors/Autorin zugeordnet!


Abstract:
Cancer stem cells or tumour-propagating cells (TPCs) have been identified for a number of cancers, but data pertaining to their existence in lymphoma so far remain elusive. We show for the first time that a small subset of cells purified from human anaplastic lymphoma kinase (ALK)-positive and -negative, anaplastic large cell lymphoma cell lines and primary patient tumours using the side population (SP) technique have serial tumour-propagating capacity both in vitro and in vivo; they give rise to both themselves and the bulk tumour population as well as supporting growth of the latter through the production of soluble factors. In vivo serial dilution assays utilising a variety of model systems inclusive of human cell lines, primary human tumours and nucleophosmin (NPM)-ALK-induced murine tumours demonstrate the TPC frequency to vary from as many as 1/54 to 1/1336 tumour cells. In addition, the SP cells express higher levels of pluripotency-associated transcription factors and are enriched for a gene expression profile consistent with early thymic progenitors. Finally, our data show that the SP cells express higher levels of the NPM-ALK oncogene and are sensitive to an ALK inhibitor.

Keywords Pubmed: Adult
Aged, 80 and over
Animals
Antineoplastic Agents, Phytogenic/pharmacology
Apoptosis
Cell Line, Tumor
Cell Proliferation
Cell Survival
Child
Child, Preschool
Etoposide/pharmacology
Female
Gene Expression Profiling
Humans
Lymphoma, Large-Cell, Anaplastic/genetics
Lymphoma, Large-Cell, Anaplastic/pathology*
Mice
Mice, Inbred C57BL
Middle Aged
Neoplastic Stem Cells/cytology
Nuclear Proteins/metabolism*
Pluripotent Stem Cells/cytology
Protein Kinase Inhibitors/pharmacology
Proto-Oncogene Proteins c-kit/biosynthesis
Pyrazoles/pharmacology
Pyridines/pharmacology
Receptor Protein-Tyrosine Kinases/antagonists & inhibitors
Receptor Protein-Tyrosine Kinases/metabolism*
Side-Population Cells/cytology*
Side-Population Cells/metabolism*
Signal Transduction


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