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Gewählte Publikation:

Publikationstyp: Zeitschriftenaufsatz
Dokumenttyp: Originalarbeit

Jahr: 1985

AutorInnen: Niedermüller, H; Hofecker, G; Skalicky, M

Titel: Changes of DNA repair mechanisms during the aging of the rat.

Quelle: Mech Ageing Dev. 1985; 29(3):221-238



Autor/innen der Vetmeduni Vienna:

Hofecker Gerhard
Niedermüller Hans
Skalicky Monika

Beteiligte Vetmed-Organisationseinheiten
Institut für Physiologie, Pathophysiologie und Biophysik, Abteilung für Physiologie, Pathophysiologie und experimentelle Endokrinologie


Abstract:
Recent studies have shown a significant reduction in DNA repair capacity in aging rats. Therefore we were interested in investigating which repair mechanism is concerned in this reduction. We investigated: excision repair (ER); single-strand break repair (SSBR); double-strand break repair (DSBR); and gamma-endonuclease susceptibility (ES) by means of the following methods: [3H]thymidine ([3H]dThd) incorporation into DNA after damage by N-methyl-N-nitrosourea (MNU); nucleoid sedimentation after damage by methyl methanesulfonate (MMS); neutral elution techniques after damage by 4-nitroquinoline-1-oxide (NQO); and determination of ES sites by velocity sedimentation in an alkaline sucrose gradient after damage by gamma-irradiation. Studies were done with male Sprague-Dawley rats aged 9, 18 and 28 months using nine different organs. We were able to determine a distinct age dependency of excision repair, a slight reduction of single-strand break repair, an elevation of gamma-endonuclease susceptible sites and no significant change in double-strand break repair in the course of aging. Therefore we see a shift in the pattern of DNA repair: in old age strand break repair mechanisms become more important, while repair replication is reduced. From this we can conclude that genetic expression is altered during the aging process, with all the consequences for the disposition toward certain diseases.

Keywords Pubmed: 4-Nitroquinoline-1-oxide/diagnostic use
Aging*
Animals
Biomechanical Phenomena
DNA/physiology
DNA/radiation effects
DNA Repair*
DNA, Single-Stranded/physiology
Drug Resistance
Endonucleases/pharmacology
Kinetics
Male
Methyl Methanesulfonate/diagnostic use
Methylnitrosourea/diagnostic use
Rats
Rats, Inbred Strains


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