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Gewählte Publikation:

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Publikationstyp: Zeitschriftenaufsatz
Dokumenttyp: Originalarbeit

Jahr: 2016

AutorInnen: Malcolm, TI; Villarese, P; Fairbairn, CJ; Lamant, L; Trinquand, A; Hook, CE; Burke, GA; Brugières, L; Hughes, K; Payet, D; Merkel, O; Schiefer, AI; Ashankyty, I; Mian, S; Wasik, M; Turner, M; Kenner, L; Asnafi, V; Macintyre, E; Turner, SD

Titel: Anaplastic large cell lymphoma arises in thymocytes and requires transient TCR expression for thymic egress.

Quelle: Nat Commun. 2016; 7:10087



Autor/innen der Vetmeduni Vienna:

Kenner Lukas

Beteiligte Vetmed-Organisationseinheiten
Institut für Pathologie, Abteilung für Labortierpathologie


Abstract:
Anaplastic large cell lymphoma (ALCL) is a peripheral T-cell lymphoma presenting mostly in children and young adults. The natural progression of this disease is largely unknown as is the identity of its true cell of origin. Here we present a model of peripheral ALCL pathogenesis where the malignancy is initiated in early thymocytes, before T-cell receptor (TCR) β-rearrangement, which is bypassed in CD4/NPM-ALK transgenic mice following Notch1 expression. However, we find that a TCR is required for thymic egress and development of peripheral murine tumours, yet this TCR must be downregulated for T-cell lymphomagenesis. In keeping with this, clonal TCR rearrangements in human ALCL are predominantly in-frame, but often aberrant, with clonal TCRα but no comparable clonal TCRβ rearrangement, yielding events that would not normally be permissive for survival during thymic development. Children affected by ALCL may thus harbour thymic lymphoma-initiating cells capable of seeding relapse after chemotherapy.

Keywords Pubmed: Adult
Animals
CD4 Antigensmetabolism
Cell Line, Tumor
Child
Disease Models, Animal
Female
Flow Cytometry
Gene Rearrangement, T-Lymphocyte
Genes, RAG-1genetics
Genes, T-Cell Receptor alpha
Genes, T-Cell Receptor beta
Humans
Immunohistochemistry
Jurkat Cells
Lymphoma, Large-Cell, Anaplasticmetabolism
Male
Mice
Mice, Transgenic
Protein-Tyrosine Kinasesmetabolism
Receptor, Notch1metabolism
Receptors, Antigen, T-Cellmetabolism
Receptors, Antigen, T-Cell, alpha-betageneticsmetabolism
Reverse Transcriptase Polymerase Chain Reaction
Thymocytesmetabolism
Thymus Glandcytology

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