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Publikationstyp: Zeitschriftenaufsatz
Dokumenttyp: Originalarbeit

Jahr: 2016

AutorInnen: Saleh, M; Kumar, G; Abdel-Baki, AA; Dkhil, MA; El-Matbouli, M; Al-Quraishy, S

Titel: In Vitro Gene Silencing of the Fish Microsporidian Heterosporis saurida by RNA Interference.

Quelle: Nucleic Acid Ther. 2016; 26(4):250-256



Autor/innen der Vetmeduni Vienna:

El-Matbouli Mansour
Kumar Gokhlesh
Saleh Mona

Beteiligte Vetmed-Organisationseinheiten
Universitätsklinik für Geflügel und Fische, Klinische Abteilung für Fischmedizin


Abstract:
Heterosporis saurida, a microsporidian parasite of lizardfish, Saurida undosquamis, causes severe economic losses in marine aquaculture. Among the novel approaches being explored for treatment of parasitic infections in aquaculture is small interfering RNA molecules. The aim of the present study was to investigate the efficiency of using siRNA to knock down expression of specific genes of H. saurida in vitro. For this purpose, siRNAs specific for ATP/ADP antiporter 1 and methionine aminopeptidase II genes were designed and tested using a previously developed in vitro cultivation model. Silencing of H. saurida target genes was assessed and the efficacy of using siRNA for inhibition of gene expression was measured by quantitative real-time polymerase chain reaction (PCR). Silencing of ATP/ADP antiporter 1 or methionine aminopeptidase II by siRNA reduced H. saurida infection levels in EK-1 cells 40% and 60%, respectively, as measured by qRT-PCR and spore counts. Combined siRNA treatment of both ATP/ADP antiporter 1 and methionine aminopeptidase II siRNAs was more effective against H. saurida infection as seen by the 16S rRNA level and spore counts. Our study concluded that siRNA could be used to advance development of novel approaches to inhibit H. saurida and provide an alternative approach to combat microsporidia.

Keywords Pubmed: Aminopeptidasesantagonists & inhibitorsgenetics
Animals
Antiportersantagonists & inhibitorsgenetics
Fish Diseasesgeneticsmicrobiologyprevention & control
Fishesgeneticsmicrobiology
Gene Silencing
Metalloendopeptidasesantagonists & inhibitorsgenetics
Microsporidiapathogenicity
RNA Interference
RNA, Small Interferinggeneticspharmacology

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