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Gewählte Publikation:

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Publikationstyp: Zeitschriftenaufsatz
Dokumentart: Short Communication

Publikationsjahr: 2016

AutorInnen: Kidane, FA; Bilic, I; Mitra, T; Wernsdorf, P; Hess, M; Liebhart, D

Titel: In situ hybridization to detect and localize signature cytokines of T-helper (Th) 1 and Th2 immune responses in chicken tissues.

Quelle: Vet Immunol Immunopathol. 2016; 175:51-56



Autor/innen der Vetmeduni Vienna:

Bilic Ivana
Hess Michael
Liebhart Dieter
Mitra Taniya
Wernsdorf Patricia

Beteiligte Vetmed-Organisationseinheiten
Klinische Abteilung für Geflügelmedizin


Zugehörige(s) Projekt(e): Immunreaktion gegen Histomonaden des Geflügels, ein Th2-Infektionsmodell?


Abstract:
The avian immune system has been shown to possess a repertoire of cytokines directing T-helper (Th) 1 and Th2 types of immune responses similar to that in mammals. The objective of this study was to establish in situ hybridization (ISH) for the localization of mRNA of selected signal cytokines, chicken interferon-γ (ChIFN-γ), chicken interleukin (ChIL)-4 and ChIL-13 in fixed tissues. RNA probes were generated to hybridize to 488, 318, and 417bp of the respective target mRNA. Probe concentrations ranging from 100ng/ml to 400ng/ml were shown to be suitable to label cells that expressed these cytokines. The specificity of every probe was verified using the respective sense probe. ChIFN-γ, ChIL-4 and ChIL-13 positive cells were observed in the lymphocytic infiltrations of liver and in the periarteriolar lymphatic sheaths of spleen collected from specific-pathogen-free chickens. ISH of these cytokines in a severely inflamed liver due to infiltration with the parasite Histomonas meleagridis revealed the expression of both ChIFN-γ and ChIL-13 mRNA in the mononuclear infiltrates. In conclusion, ChIFN-γ, ChIL-4 and ChIL-13 mRNA were efficiently localized by ISH, which supplies a valid technique to characterize immune responses in fixed tissues.

Keywords Pubmed: Animals
Avian Proteins/genetics
Chickens/genetics*
Chickens/immunology*
Cytokines/genetics*
Gene Expression
In Situ Hybridization
Interferon-gamma/genetics
Interleukin-13/genetics
Interleukin-4/genetics
Liver/cytology
Liver/immunology
RNA, Messenger/genetics
Specific Pathogen-Free Organisms/genetics
Specific Pathogen-Free Organisms/immunology
Spleen/cytology
Spleen/immunology
Th1 Cells/immunology*
Th2 Cells/immunology*


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