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Publikationstyp: Zeitschriftenaufsatz
Dokumentart: Originalarbeit

Publikationsjahr: 2016

AutorInnen: Qumar, M; Khiaosa-Ard, R; Pourazad, P; Wetzels, SU; Klevenhusen, F; Kandler, W; Aschenbach, JR; Zebeli, Q

Titel: Evidence of In Vivo Absorption of Lactate and Modulation of Short Chain Fatty Acid Absorption from the Reticulorumen of Non-Lactating Cattle Fed High Concentrate Diets.

Quelle: PLoS One. 2016; 11(10):e0164192



Autor/innen der Vetmeduni Vienna:

Khiaosa-Ard Ratchaneewan
Klevenhusen Fenja
Pourazad Poulad
Qumar Muhammad
Wetzels Stefanie
Zebeli Qendrim

Beteiligte Vetmed-Organisationseinheiten
Institut für Lebensmittelsicherheit, Lebensmitteltechnologie und öffentliches Gesundheitswesen in der Veterinärmedizin, Abteilung für Lebensmittelmikrobiologie
Institut für Tierernährung und funktionelle Pflanzenstoffe


Zugehörige(s) Projekt(e): d-i.INFLACOW: Characterization and prevention of diet-induced inflammation and related immune and metabolic disorders in dairy cows


Abstract:
Short-chain fatty acids (SCFAs) and lactate are endproducts of rumen fermentation and important energy sources for the host ruminant. Because their rapid accumulation results in ruminal acidosis, enhancement of the absorption of SCFA and lactate across reticuloruminal wall is instrumental in increasing energy supply and preventing ruminal acidosis in cattle. This study investigated whether the reticuloruminal absorption of SCFAs and lactate was altered by different strategies of high concentrate feeding. Eight rumen-cannulated, non-lactating Holstein cows were fed a forage-only diet (baseline) and then gradually adapted over 6 d to a 60% concentrate level. Thereafter, this concentrate-rich diet was fed for 4 wk either continuously (Con; n = 8) or interruptedly (Int; n = 8). Absorption of SCFAs and lactate was determined in vivo from the experimental buffer introduced into the washed reticulorumen. The buffer contained acetate, propionate, butyrate and lactate at a concentration of 60, 30, 10 and 5 mmol/L, respectively and Cr-EDTA as a marker for correcting ruminal water fluxes. The reticuloruminal absorption after 35 and 65 min of buffer incubation was measured at the baseline, after 1 wk of 60% concentrate feeding in the interrupted model (Int-1) and after 4 wk of concentrate feeding in both feeding models (Int-4 and Con-4). Data showed that the absorption rates of individual and total SCFAs during the first 35 min of incubation of Con-4 were highest (~1.7 times compared to baseline), while Int-1 and Int-4 were similar to respective baseline. Lactate was not absorbed during forage-only baseline and 1-wk concentrate feeding, but after 4-wk feeding of concentrates in both models. In conclusion, SCFAs absorption across the reticulorumen of non-lactating cattle was enhanced by the 4-wk continuous concentrate feeding, which seems to be more advantageous in terms of rumen acidosis prevention compared to the interrupted feeding model. The study provides evidence of lactate absorption across the reticulorumen of non-lactating cattle after both continuous and interrupted 4-wk concentrate feeding.

Keywords Pubmed: Acidosis/metabolism
Animal Feed
Animal Nutritional Physiological Phenomena/physiology
Animals
Cattle
Cross-Over Studies
Diet
Fatty Acids, Volatile/metabolism*
Feeding Behavior/physiology
Female
Lactation/metabolism
Lactic Acid/metabolism*
Rumen/metabolism*


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