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Publikationstyp: Zeitschriftenaufsatz
Dokumentart: Originalarbeit

Publikationsjahr: 2016

AutorInnen: Mitra, T; Bilic, I; Hess, M; Liebhart, D

Titel: The 60S ribosomal protein L13 is the most preferable reference gene to investigate gene expression in selected organs from turkeys and chickens, in context of different infection models.

Quelle: Vet Res. 2016; 47(1):105



Autor/innen der Vetmeduni Vienna:

Bilic Ivana
Hess Michael
Liebhart Dieter
Mitra Taniya

Beteiligte Vetmed-Organisationseinheiten
Klinische Abteilung für Geflügelmedizin


Zugehörige(s) Projekt(e): Immunreaktion gegen Histomonaden des Geflügels, ein Th2-Infektionsmodell?


Abstract:
Evaluation of reference genes for expression studies in chickens and turkeys is very much limited and unavailable for various infectious models. In this study, eight candidate reference genes HMBS, HPRT1, TBP, VIM, TFRC, RPLP0, RPL13 and RPS7 were evaluated by five different algorithms (GeNorm, NormFinder, BestKeeper©, delta CT, RefFinder) to assess their stability. In order to analyze a broad variation of tissues, spleen, liver, caecum and caecal tonsil of different aged specific pathogen free (SPF) layer chickens and commercial turkeys, uninfected or infected with the extracellular pathogen Histomonas meleagridis, were included. For tissue samples from SPF chickens RPL13 and TBP were found to be the most stable reference genes. Further testing of RPL13 and TBP in the same organs of uninfected and infected SPF broiler chickens with the intracellular pathogen fowl aviadenovirus confirmed this finding. In tissue samples from turkeys, a stable expression of RPL13 and TFRC genes was noticed. Overall, the determined reference genes should be considered whenever gene expression studies in spleen, liver, caecum and caecal tonsil of chickens and turkeys are performed.

Keywords Pubmed: Animals
Chickens/genetics
Chickens/metabolism*
Female
Gene Expression/genetics*
Gene Expression Profiling/veterinary
Genes/genetics
Poultry Diseases/genetics
Poultry Diseases/metabolism
Poultry Diseases/parasitology
Protozoan Infections, Animal/metabolism
RNA/genetics
RNA/isolation & purification
Real-Time Polymerase Chain Reaction/veterinary
Ribosomal Proteins/genetics
Ribosomal Proteins/metabolism*
Trichomonadida
Turkeys/genetics
Turkeys/metabolism*


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