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Publikationstyp: Zeitschriftenaufsatz
Dokumentart: Originalarbeit

Publikationsjahr: 2017

AutorInnen: Sassu, EL; Ladinig, A; Talker, SC; Stadler, M; Knecht, C; Stein, H; Frömbling, J; Richter, B; Spergser, J; Ehling-Schulz, M; Graage, R; Hennig-Pauka, I; Gerner, W

Titel: Frequency of Th17 cells correlates with the presence of lung lesions in pigs chronically infected with Actinobacillus pleuropneumoniae.

Quelle: Vet Res. 2017; 48(1):4



Autor/innen der Vetmeduni Vienna:

Ehling-Schulz Monika
Frömbling Janna
Gerner Wilhelm
Hennig-Pauka Isabel
Knecht Christian
Ladinig Andrea
Richter Barbara
Sassu Elena Lucia
Spergser Joachim
Stadler Maria
Stein Heiko
Talker Stephanie

Beteiligte Vetmed-Organisationseinheiten
Abteilung für Funktionelle Mikrobiologie
Institut für Immunologie
Institut für Mikrobiologie
Institut für Pathologie
Universitätsklinik für Schweine


Abstract:
Porcine contagious pleuropneumonia caused by Actinobacillus pleuropneumoniae (APP) remains one of the major causes of poor growth performance and respiratory disease in pig herds. While the role of antibodies against APP has been intensely studied, the porcine T cell response remains poorly characterized. To address this, pigs were intranasally infected with APP serotype 2 and euthanized during the acute phase [6-10 days post-infection (dpi)] or the chronic phase of APP infection (27-31 dpi). Lymphocytes isolated from blood, tonsils, lung tissue and tracheobronchial lymph nodes were analyzed by intracellular cytokine staining (ICS) for IL-17A, IL-10 and TNF-α production after in vitro stimulation with crude capsular extract (CCE) of the APP inoculation strain. This was combined with cell surface staining for the expression of CD4, CD8α and TCR-γδ. Clinical records, microbiological investigations and pathological findings confirmed the induction of a subclinical APP infection. ICS-assays revealed the presence of APP-CCE specific CD4(+)CD8α(dim) IL-17A-producing T cells in blood and lung tissue in most infected animals during the acute and chronic phase of infection and a minor fraction of these cells co-produced TNF-α. APP-CCE specific IL-17A-producing γδ T cells could not be found and APP-CCE specific IL-10-producing CD4(+) T cells were present in various organs but only in a few infected animals. The frequency of identified putative Th17 cells (CD4(+)CD8α(dim)IL-17A(+)) in lung and blood correlated positively with lung lesion scores and APP-specific antibody titers during the chronic phase. These results suggest a potential role of Th17 cells in the immune pathogenesis of APP infection.

Keywords Pubmed: Actinobacillus Infections/immunology
Actinobacillus Infections/microbiology
Actinobacillus Infections/pathology
Actinobacillus Infections/veterinary*
Actinobacillus pleuropneumoniae*/immunology
Animals
Chronic Disease
Lung/immunology
Lung/microbiology
Lung/pathology*
Lymph Nodes/pathology
Male
Pleuropneumonia/immunology
Pleuropneumonia/microbiology
Pleuropneumonia/pathology
Pleuropneumonia/veterinary*
Swine
Swine Diseases/immunology
Swine Diseases/microbiology*
Swine Diseases/pathology
Th17 Cells/pathology*


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