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Publikationstyp: Zeitschriftenaufsatz
Dokumenttyp: Originalarbeit

Jahr: 2017

AutorInnen: Kumar, G; Hummel, K; Welch, TJ; Razzazi-Fazeli, E; El-Matbouli, M

Titel: Global proteomic profiling of Yersinia ruckeri strains.

Quelle: Vet Res. 2017; 48(1):55



Autor/innen der Vetmeduni Vienna:

El-Matbouli Mansour
Hummel Karin
Kumar Gokhlesh
Razzazi-Fazeli Ebrahim

Beteiligte Vetmed-Organisationseinheiten
Universitätsklinik für Geflügel und Fische, Klinische Abteilung für Fischmedizin
VetCore


Zugehörige(s) Projekt(e): Proteomik der Regenbogenforelle als Antwort auf Y. ruckeri


Abstract:
Yersinia ruckeri is the causative agent of enteric redmouth disease (ERM) of salmonids. There is little information regarding the proteomics of Y. ruckeri. Herein, we perform whole protein identification and quantification of biotype 1 and biotype 2 strains of Y. ruckeri grown under standard culture conditions using a shotgun proteomic approach. Proteins were extracted, digested and peptides were separated by a nano liquid chromatography system and analyzed with a high-resolution hybrid triple quadrupole time of flight mass spectrometer coupled via a nano ESI interface. SWATH-MS technology and sophisticated statistical analyses were used to identify proteome differences among virulent and avirulent strains. GO annotation, subcellular localization, virulence proteins and antibiotic resistance ontology were predicted using bioinformatic tools. A total of 1395 proteins were identified in the whole cell of Y. ruckeri. These included proteases, chaperones, cell division proteins, outer membrane proteins, lipoproteins, receptors, ion binding proteins, transporters and catalytic proteins. In virulent strains, a total of 16 proteins were upregulated including anti-sigma regulatory factor, arginine deiminase, phosphate-binding protein PstS and superoxide dismutase Cu-Zu. Additionally, several virulence proteins were predicted such as Clp and Lon pro-teases, TolB, PPIases, PstS, PhoP and LuxR family transcriptional regulators. These putative virulence proteins might be used for development of novel targets for treatment of ERM in fish. Our study represents one of the first global proteomic reference profiles of Y. ruckeri and this data can be accessed via ProteomeXchange with identifier PXD005439. These proteomic profiles elucidate proteomic mechanisms, pathogenicity, host-interactions, antibiotic resistance ontology and localization of Y. ruckeri proteins.

Keywords Pubmed: Animals
Anti-Bacterial Agentspharmacology
Bacterial Proteinsgeneticsisolation & purification
Drug Resistance, Bacterialgenetics
Fish Diseasesmicrobiology
Gene Ontology
Proteomicsmethods
Yersinia Infectionsmicrobiologyveterinary
Yersinia ruckeridrug effectsgenetics

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