Veterinärmedizinische Universität Wien Forschungsinformationssystem VetDoc

Grafischer Link zur Startseite der Vetmeduni Vienna

Gewählte Publikation:

Publikationstyp: Zeitschriftenaufsatz
Dokumentart: Originalarbeit

Publikationsjahr: 2017

AutorInnen: Hykollari, A; Malzl, D; Yan, S; Wilson, IBH; Paschinger, K

Titel: Hydrophilic interaction anion exchange for separation of multiply modified neutral and anionic Dictyostelium N-glycans.

Quelle: Electrophoresis. 2017; 38(17):2175-2183



Autor/innen der Vetmeduni Vienna:

Yan Shi

Diese Publikation wurde nicht im Namen der Vetmeduni Vienna erstellt und ist deshalb ausschließlich der persönlichen Publikationsliste des/der Autors/Autorin zugeordnet!


Abstract:
The unusual nature of the N-glycans of the cellular slime mould Dictyostelium discoideum has been revealed by a number of studies, primarily based on examination of radiolabeled glycopeptides but more recently also by MS. The complexity of the N-glycomes of even glycosylation mutants is compounded by the occurrence of anionic modifications, which also present an analytical challenge. In this study, we have employed hydrophilic interaction anion exchange (HIAX) HPLC in combination with MALDI-TOF MS/MS to explore the anionic N-glycome of the M31 (modA) strain, which lacks endoplasmic reticulum α-glucosidase II, an enzyme conserved in most eukaryotes including Homo sapiens. Prefractionation with HIAX chromatography enabled the identification of N-glycans with unusual oligo-α1,2-mannose extensions as well as others with up to four anionic modifications. Due to the use of hydrofluoric acid treatment, we were able to discriminate isobaric glycans differing in the presence of sulphate or phosphate on intersected structures as opposed to those carrying GlcNAc-phosphodiesters. The latter represent biosynthetic intermediates during the pathway leading to formation of the methylphosphorylated mannose epitope, which may have a similar function in intracellular targeting of hydrolases as the mannose-6-phosphate modification of lysosomal enzymes in mammals. In conclusion, HIAX in combination with MS is a highly sensitive approach for both fine separation and definition of neutral and anionic N-glycan structures.

Keywords Pubmed: Chromatography, High Pressure Liquid/methods
Chromatography, Ion Exchange/methods*
Dictyostelium/chemistry*
Dictyostelium/metabolism
Glycomics/methods*
Hexoses/analysis
Hexoses/chemistry
Hydrophobic and Hydrophilic Interactions
Mannose/analysis
Mannose/chemistry
Phosphates/analysis
Phosphates/chemistry
Polysaccharides/analysis*
Polysaccharides/chemistry*
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods
Sulfates/analysis
Sulfates/chemistry


© Veterinärmedizinische Universität Wien Hilfe und Downloads