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Gewählte Publikation:

Publikationstyp: Zeitschriftenaufsatz
Dokumenttyp: Originalarbeit

Jahr: 2015

AutorInnen: Yan, S; Brecker, L; Jin, C; Titz, A; Dragosits, M; Karlsson, NG; Jantsch, V; Wilson, IB; Paschinger, K

Titel: Bisecting Galactose as a Feature of N-Glycans of Wild-type and Mutant Caenorhabditis elegans.

Quelle: Mol Cell Proteomics. 2015; 14(8):2111-2125



Autor/innen der Vetmeduni Vienna:

Yan Shi

Diese Publikation wurde nicht im Namen der Vetmeduni Vienna erstellt und ist deshalb ausschließlich der persönlichen Publikationsliste des/der Autors/Autorin zugeordnet!


Abstract:
The N-glycosylation of the model nematode Caenorhabditis elegans has proven to be highly variable and rather complex; it is an example to contradict the existing impression that "simple" organisms possess also a rather simple glycomic capacity. In previous studies in a number of laboratories, N-glycans with up to four fucose residues have been detected. However, although the linkage of three fucose residues to the N,N"-diacetylchitobiosyl core has been proven by structural and enzymatic analyses, the nature of the fourth fucose has remained uncertain. By constructing a triple mutant with deletions in the three genes responsible for core fucosylation (fut-1, fut-6 and fut-8), we have produced a nematode strain lacking products of these enzymes, but still retaining maximally one fucose residue on its N-glycans. Using mass spectrometry and HPLC in conjunction with chemical and enzymatic treatments as well as NMR, we examined a set of α-mannosidase-resistant N-glycans. Within this glycomic subpool, we can reveal that the core β-mannose can be trisubstituted and so carries not only the ubiquitous α1,3- and α1,6-mannose residues, but also a "bisecting" β-galactose, which is substoichiometrically modified with fucose or methylfucose. In addition, the α1,3-mannose can also be α-galactosylated. Our data, showing the presence of novel N-glycan modifications, will enable more targeted studies to understand the biological functions and interactions of nematode glycans.

Keywords Pubmed: Animals
Caenorhabditis elegans/metabolism*
Chromatography, High Pressure Liquid
Chromatography, Reverse-Phase
Fucose/metabolism
Fucosyltransferases/metabolism
Galactose/metabolism*
Gene Knockout Techniques
Glycoproteins/metabolism
Isomerism
Mannosidases/metabolism
Methylation
Mutation/genetics*
Polysaccharides/chemistry
Polysaccharides/metabolism*
Protein Isoforms/metabolism
Proteome/metabolism
Proton Magnetic Resonance Spectroscopy
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Tandem Mass Spectrometry


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