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Gewählte Publikation:

Publikationstyp: Zeitschriftenaufsatz
Dokumenttyp: Originalarbeit

Jahr: 2011

AutorInnen: Serna, S; Yan, S; Martin-Lomas, M; Wilson, IB; Reichardt, NC

Titel: Fucosyltransferases as synthetic tools: glycan array based substrate selection and core fucosylation of synthetic N-glycans.

Quelle: J Am Chem Soc. 2011; 133(41):16495-16502



Autor/innen der Vetmeduni Vienna:

Yan Shi

Diese Publikation wurde nicht im Namen der Vetmeduni Vienna erstellt und ist deshalb ausschließlich der persönlichen Publikationsliste des/der Autors/Autorin zugeordnet!


Abstract:
Two recombinant fucosyltransferases were employed as synthetic tools in the chemoenzymatic synthesis of core fucosylated N-glycan structures. Enzyme substrates were rapidly identified by incubating a microarray of synthetic N-glycans with the transferases and detecting the presence of core fucose with four lectins and one antibody. Selected substrates were then enzymatically fucosylated in solution on a preparative scale and characterized by NMR and MS. With this approach the chemoenzymatic synthesis of a series of α1,3-, α1,6-, and difucosylated structures was accomplished in very short time and with high yields, which otherwise would have required extensive additional synthetic effort and a complete redesign of existing synthetic routes. In addition, valuable information was gathered regarding the specificities of the lectins employed in this study.

Keywords Pubmed: Carbohydrate Conformation
Fucosyltransferases/chemistry
Fucosyltransferases/metabolism*
Microarray Analysis*
Molecular Sequence Data
Polysaccharides/biosynthesis*
Polysaccharides/chemistry
Substrate Specificity


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