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Publikationstyp: Zeitschriftenaufsatz
Dokumenttyp: Originalarbeit

Jahr: 2018

AutorInnen: Soliman, H; Kumar, G; El-Matbouli, M

Titel: Recombinase polymerase amplification assay combined with a lateral flow dipstick for rapid detection of Tetracapsuloides bryosalmonae, the causative agent of proliferative kidney disease in salmonids.

Quelle: Parasit Vectors. 2018; 11(1):234

Autor/innen der Vetmeduni Vienna:

El-Matbouli Mansour
Kumar Gokhlesh
Soliman Hatem

Beteiligte Vetmed-Organisationseinheiten
Universitätsklinik für Geflügel und Fische, Klinische Abteilung für Fischmedizin

Zugehörige(s) Projekt(e): In vivo induzierte Gene von Tetracapsuloides bryosalmonae

The myxozoan Tetracapsuloides bryosalmonae, the causative agent of proliferative kidney disease (PKD), is responsible for considerable losses in farmed and wild fish populations in Europe and North America. Recently, T. bryosalmonae was detected in many European countries, and strategy to control the disease in the wild and farmed fish population is yet to be developed. Recombinase polymerase amplification (RPA) is a novel isothermal nucleic acid amplification technology that does not require any thermal cycling, and lateral flow dipstick (LFD) is a rapid, cost-effective, and easy-to-handle assay that enables stable detection.In this study, we developed and optimized a rapid and sensitive RPA assay combined with an LFD for the detection of T. bryosalmonae. The PKD-RPA assay was specific to T. bryosalmonae, as no cross-reaction or false positive signals were observed with any of the other tested DNAs. The developed PKD-RPA assay was ten times more sensitive than an existing diagnostic polymerase chain reaction (PCR) assay for this parasite. The estimated time to perform PKD-RPA assay is 25 min compared to 4 h for PKD-PCR assay.A novel PKD-RPA assay for the detection of T. bryosalmonae was developed. The assay offers considerable advantages including speed, sensitivity, specificity and visual detection. Applying the PKD-RPA assay combined with an LFD enhances the surveillance and early detection of T. bryosalmonae in salmonids.

Keywords Pubmed: Animals
Chromatography, Affinitymethods
Fish Diseasesdiagnosisparasitology
Kidney Diseasesdiagnosisparasitologyveterinary
Myxozoaisolation & purification
Nucleic Acid Amplification Techniquesmethods
Parasitic Diseases, Animaldiagnosisparasitology
Sensitivity and Specificity
Time Factors

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