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Gewählte Publikation:

Publikationstyp: Zeitschriftenaufsatz
Dokumentart: Originalarbeit

Publikationsjahr: 2016

AutorInnen: Koller, A; Rid, R; Beyreis, M; Bianchini, R; Holub, BS; Lang, A; Locker, F; Brodowicz, B; Velickovic, O; Jakab, M; Kerschbaum, H; Önder, K; Kofler, B

Titel: In vitro toxicity of the galanin receptor 3 antagonist SNAP 37889.

Quelle: Neuropeptides. 2016; 56:83-88



Autor/innen der Vetmeduni Vienna:

Locker Felix

Diese Publikation wurde nicht im Namen der Vetmeduni Vienna erstellt und ist deshalb ausschließlich der persönlichen Publikationsliste des/der Autors/Autorin zugeordnet!


Abstract:
Galanin and its receptors (GAL1, GAL2, GAL3) modulate a range of neuronal, immune and vascular activities. In vivo administration of SNAP 37889 (1-phenyl-3-[[3-(trifluoromethyl)phenyl]imino]-1H-indol-2-one), a potent small non-peptidergic antagonist of GAL3, was reported to reduce anxiety- and depression-related behavior, ethanol consumption, and antagonizes the effect of galanin on plasma extravasation in rodent models. Accordingly, SNAP 37889 has been proposed as a potential therapeutic agent to treat anxiety and depression disorders. Therefore, we evaluated the toxicity of SNAP 37889 to different cell types. Our experiments revealed that SNAP 37889 (≥10μM) induced apoptosis in epithelial (HMCB) and microglial (BV-2) cell lines expressing endogenous GAL3, in peripheral blood mononuclear cells and promyelocytic leukemia cells (HL-60) expressing GAL2, and in a neuronal cell line (SH-SY5Y) lacking galanin receptor expression altogether. In conclusion, SNAP 37889 is toxic to a variety of cell types independent of GAL3 expression. We caution that the clinical use of SNAP 37889 at doses that might be used to treat anxiety- or depression- related diseases could have unexpected non-galanin receptor-mediated toxicity, especially on immune cells.

Keywords Pubmed: Animals
Apoptosis/drug effects*
Cell Line
Cell Line, Tumor
Cell Survival/drug effects
Epithelial Cells/drug effects
Humans
In Vitro Techniques
Indoles/toxicity*
Leukocytes, Mononuclear/drug effects
Mice
Microglia/drug effects
Neurons/drug effects
Receptor, Galanin, Type 3/antagonists & inhibitors*


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