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Gewählte Publikation:

Publikationstyp: Zeitschriftenaufsatz
Dokumenttyp: Originalarbeit

Jahr: 2016

AutorInnen: Koller, A; Rid, R; Beyreis, M; Bianchini, R; Holub, BS; Lang, A; Locker, F; Brodowicz, B; Velickovic, O; Jakab, M; Kerschbaum, H; Önder, K; Kofler, B

Titel: In vitro toxicity of the galanin receptor 3 antagonist SNAP 37889.

Quelle: Neuropeptides. 2016; 56:83-88



Autor/innen der Vetmeduni Vienna:

Bianchini Rodolfo
Locker Felix

Diese Publikation wurde nicht im Namen der Vetmeduni Vienna erstellt und ist deshalb ausschließlich der persönlichen Publikationsliste des/der Autors/Autorin zugeordnet!


Abstract:
Galanin and its receptors (GAL1, GAL2, GAL3) modulate a range of neuronal, immune and vascular activities. In vivo administration of SNAP 37889 (1-phenyl-3-[[3-(trifluoromethyl)phenyl]imino]-1H-indol-2-one), a potent small non-peptidergic antagonist of GAL3, was reported to reduce anxiety- and depression-related behavior, ethanol consumption, and antagonizes the effect of galanin on plasma extravasation in rodent models. Accordingly, SNAP 37889 has been proposed as a potential therapeutic agent to treat anxiety and depression disorders. Therefore, we evaluated the toxicity of SNAP 37889 to different cell types. Our experiments revealed that SNAP 37889 (≥10μM) induced apoptosis in epithelial (HMCB) and microglial (BV-2) cell lines expressing endogenous GAL3, in peripheral blood mononuclear cells and promyelocytic leukemia cells (HL-60) expressing GAL2, and in a neuronal cell line (SH-SY5Y) lacking galanin receptor expression altogether. In conclusion, SNAP 37889 is toxic to a variety of cell types independent of GAL3 expression. We caution that the clinical use of SNAP 37889 at doses that might be used to treat anxiety- or depression- related diseases could have unexpected non-galanin receptor-mediated toxicity, especially on immune cells. Copyright © 2015 Elsevier Ltd. All rights reserved.

Keywords Pubmed: Animals
Apoptosisdrug effects
Cell Line
Cell Line, Tumor
Cell Survivaldrug effects
Epithelial Cellsdrug effects
Humans
In Vitro Techniques
Indolestoxicity
Leukocytes, Mononucleardrug effects
Mice
Microgliadrug effects
Neuronsdrug effects
Receptor, Galanin, Type 3antagonists & inhibitors

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