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Publikationstyp: Zeitschriftenaufsatz
Dokumenttyp: Originalarbeit

Jahr: 2019

AutorInnen: Martini, F; Eckmair, B; Štefanić, S; Jin, C; Garg, M; Yan, S; Jiménez-Castells, C; Hykollari, A; Neupert, C; Venco, L; Varón Silva, D; Wilson, IBH; Paschinger, K

Titel: Highly modified and immunoactive N-glycans of the canine heartworm.

Quelle: Nat Commun. 2019; 10(1):75

Autor/innen der Vetmeduni Vienna:

Yan Shi

Beteiligte Vetmed-Organisationseinheiten
Institut für Parasitologie

Zugehörige(s) Projekt(e): Biosynthese von Wurm N-Glykoproteinen in Insektenzellen

The canine heartworm (Dirofilaria immitis) is a mosquito-borne parasitic nematode whose range is extending due to climate change. In a four-dimensional analysis involving HPLC, MALDI-TOF-MS and MS/MS in combination with chemical and enzymatic digestions, we here reveal an N-glycome of unprecedented complexity. We detect N-glycans of up to 7000 Da, which contain long fucosylated HexNAc-based repeats, as well as glucuronylated structures. While some modifications including LacdiNAc, chitobiose, α1,3-fucose and phosphorylcholine are familiar, anionic N-glycans have previously not been reported in nematodes. Glycan array data show that the neutral glycans are preferentially recognised by IgM in dog sera or by mannose binding lectin when antennal fucose and phosphorylcholine residues are removed; this pattern of reactivity is reversed for mammalian C-reactive protein, which can in turn be bound by the complement component C1q. Thereby, the N-glycans of D. immitis contain features which may either mediate immunomodulation of the host or confer the ability to avoid immune surveillance.

Keywords Pubmed: Animals
C-Reactive Proteinimmunologymetabolism
Chromatography, High Pressure Liquidmethods
Complement C1qimmunologymetabolism
Dirofilaria immitischemistryimmunology
Host-Parasite Interactionsimmunology
Immunologic Surveillanceimmunology
Protein Binding
Tandem Mass Spectrometrymethods

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