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Gewählte Publikation:

Publikationstyp: Zeitschriftenaufsatz
Dokumenttyp: Originalarbeit

Jahr: 2019

AutorInnen: Loncaric, I; Kübber-Heiss, A; Posautz, A; Ruppitsch, W; Lepuschitz, S; Schauer, B; Feßler, AT; Krametter-Frötscher, R; Harrison, EM; Holmes, MA; Künzel, F; Szostak, MP; Hauschild, T; Desvars-Larrive, A; Misic, D; Rosengarten, R; Walzer, C; Slickers, P; Monecke, S; Ehricht, R; Schwarz, S; Spergser, J

Titel: Characterization of mecC gene-carrying coagulase-negative Staphylococcus spp. isolated from various animals.

Quelle: Vet Microbiol. 2019; 230:138-144



Autor/innen der Vetmeduni Vienna:

Desvars Amelie
Krametter-Frötscher Reinhild
Kübber-Heiss Anna
Künzel Frank
Loncaric Igor
Posautz Annika
Rosengarten Renate
Spergser Joachim
Szostak Michael
Walzer Christian

Beteiligte Vetmed-Organisationseinheiten
Forschungsinstitut für Wildtierkunde und Ökologie, Conservation Medicine
Forschungsinstitut für Wildtierkunde und Ökologie
Institut für Mikrobiologie
Universitätsklinik für Kleintiere, Klinische Abteilung für Interne Medizin Kleintiere
Universitätsklinik für Wiederkäuer, Klinische Abteilung für Wiederkäuermedizin


Abstract:
The presence of the methicillin resistance gene mecC in coagulase-negative Staphylococcus spp. (CoNS) is scarce. The aim of this study was to characterize mecC-positive CoNS isolated from various wild and domestic animals. The presence of the mecC gene was screened in 4299 samples from wild animals and domestic animals. Fifteen coagulase-negative staphylococci, that displayed a cefoxitin-resistant phenotype, were tested mecC-positive by PCR. Antimicrobial susceptibility testing was performed for all isolates. The 15 isolates were genotyped by sequencing of the entire class E mec gene complex (blaZ-mecC-mecR1-mecI), the ccrA and ccrB recombinase genes and other determinants within the type XI SCCmec element. DNA microarray analysis was performed and five selected isolates were additionally whole genome sequenced and analyzed. S. stepanovicii (n = 3), S. caprae (n = 1), S. warneri (n = 1), S. xylosus (n = 1) and S. sciuri (n = 9) were detected. All but the S. sciuri isolates were found to be susceptible to all non-beta lactams. The entire class E mec gene complex was detected in all isolates but ccrA and ccrB genes were not identified in S. stepanovicii and S. xylosus. The genes erm(B) and fexA (n = 4, each) were the most predominant non-beta lactam resistance genes detected in the S. sciuri isolates. Even though the presence of the mecC gene among CoNS is a rare observation, this study further expands our knowledge by showing that the mecC gene, including its allotypes, are present in more staphylococcal species from different animal species than has been previously described.


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