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Gewählte Publikation:

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Publikationstyp: Zeitschriftenaufsatz
Dokumentart: Originalarbeit

Publikationsjahr: 2019

AutorInnen: Riedel, C; Lamp, B; Chen, HW; Heimann, M; Rümenapf, T

Titel: Fluorophore labelled BVDV: a novel tool for the analysis of infection dynamics.

Quelle: Sci Rep. 2019; 9(1):5972


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VetCore (VetImaging);

Autor/innen der Vetmeduni Vienna:

Lamp Benjamin,
Riedel Christiane,
Rümenapf Hans Tillmann,

Beteiligte Vetmed-Organisationseinheiten
Institut für Virologie,


Zugehörige(s) Projekt(e): Determination of pestivirus and surface protein structure by Cryo electron tomography


Abstract:
Genetic labelling of viruses with a fluorophore allows to study their life cycle in real time, without the need for fixation or staining techniques. Within the family Flaviviridae, options for genetic labelling of non-structural proteins exist. Yet, no system to genetically label structural proteins has been put forward to date. Taking advantage of a previously described site within the structural protein E2, a fluorophore was introduced into a cytopathogenic (cpe) BVDV-1 virus (BVDV


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