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Gewählte Publikation:

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Publikationstyp: Zeitschriftenaufsatz
Dokumenttyp: Originalarbeit

Jahr: 2019

AutorInnen: Himmel, T; Harl, J; Kübber-Heiss, A; Konicek, C; Fernández, N; Juan-Sallés, C; Ilgūnas, M; Valkiūnas, G; Weissenböck, H

Titel: Molecular probes for the identification of avian Haemoproteus and Leucocytozoon parasites in tissue sections by chromogenic in situ hybridization.

Quelle: Parasit Vectors. 2019; 12(1):282



Autor/innen der Vetmeduni Vienna:

Harl Josef
Himmel Tanja
Konicek Cornelia
Kübber-Heiss Anna
Weissenböck Herbert

Beteiligte Vetmed-Organisationseinheiten
Forschungsinstitut für Wildtierkunde und Ökologie
Institut für Pathologie
Universitätsklinik für Kleintiere, Klinische Abteilung für Interne Medizin Kleintiere


Zugehörige(s) Projekt(e): Malaria und andere Hämosporidiosen bei Wildvögeln


Abstract:
Avian haemosporidian parasites can cause severe disease in their hosts due to excessive exo-erythrocytic merogony and anaemia caused by blood stages. Notably, the development of megalomeronts by species of Haemoproteus and Leucocytozoon has been associated with mortalities in birds. Diagnosis of lethal infections is currently accomplished by the detection of parasites" tissue stages in histological sections combined with PCR and sequencing. However, sequences frequently are not reliably obtained and the generic discrimination of exo-erythrocytic tissue stages based on morphological characters is challenging. Therefore, the present study aimed at developing specific molecular probes for the identification of Haemoproteus spp. and Leucocytozoon spp. in histological sections using chromogenic in situ hybridization.


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