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Gewählte Publikation:

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Publikationstyp: Zeitschriftenaufsatz
Dokumenttyp: Originalarbeit

Jahr: 2019

AutorInnen: Harter, E; Lassnig, C; Wagner, EM; Zaiser, A; Wagner, M; Rychli, K

Titel: The Novel Internalins InlP1 and InlP4 and the Internalin-Like Protein InlP3 Enhance the Pathogenicity of Listeria monocytogenes .

Quelle: Front Microbiol. 2019; 10:1644



Autor/innen der Vetmeduni Vienna:

Kober-Rychli Kathrin
Lassnig Caroline
Wagner Eva
Wagner Eva Maria
Wagner Martin

Beteiligte Vetmed-Organisationseinheiten
Institut für Lebensmittelsicherheit, Lebensmitteltechnologie und öffentliches Gesundheitswesen in der Veterinärmedizin, Abteilung für Lebensmittelmikrobiologie
Institut für Tierzucht und Genetik, Abteilung für Molekulare Genetik


Zugehörige(s) Projekt(e): Novel internalin-like proteins

Hypervariable genetische Hotspots in Listeria monocytogenes

Österreichisches Kompetenzzentrum für Futter- und Nahrungsmittelqualität, Sicherheit und Innovation


Abstract:
The pathogenicity of the human foodborne pathogen Listeria monocytogenes relies on virulence factors such as internalins. In 2009/2010 two L. monocytogenes strains were responsible for a serious listeriosis outbreak in Austria, Germany, and the Czech Republic. One of these clones, QOC1, which caused 14 cases including five fatalities, encodes the novel internalins inlP1 , inlPq and inlP4 , and the novel internalin-like protein inlP3 in the genomic region of hypervariable genetic hotspot 9 in addition to the standard set of virulence genes. The in silico prevalence study revealed that these genes rarely occur in L. monocytogenes , mainly in minor clonal complexes. To obtain first insights of the role of these genes in the pathogenicity of L. monocytogenes , we studied the gene expression under conditions mimicking the ingestion in the host. Expression of inlP1 , inlP3 , inlPq and inlP4 was increased under gastric stress and in intracellular bacteria grown in intestinal epithelial cells. Furthermore, colonization of the liver and the spleen was slightly, but significantly reduced 72 h post infection in an oral mouse infection model when inlP1 or inlP4 was deleted. Moreover, the impact of InlP1 and InlP3 in virulence was shown in vitro in human intestinal epithelial cells. In this study we conclusively demonstrate a potential contribution of uncommon novel internalins and an internalin-like protein to the pathogenicity of L. monocytogenes .


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