Veterinärmedizinische Universität Wien Forschungsinformationssystem VetDoc

Grafischer Link zur Startseite der Vetmeduni Vienna

Gewählte Publikation:

Open Access Logo

Publikationstyp: Zeitschriftenaufsatz
Dokumenttyp: Originalarbeit

Jahr: 2019

AutorInnen: Lagler, J; Mitra, T; Schmidt, S; Pierron, A; Vatzia, E; Stadler, M; Hammer, SE; Mair, KH; Grafl, B; Wernsdorf, P; Rauw, F; Lambrecht, B; Liebhart, D; Gerner, W

Titel: Cytokine production and phenotype of Histomonas meleagridis-specific T cells in the chicken.

Quelle: Vet Res. 2019; 50(1):107



Autor/innen der Vetmeduni Vienna:

Gerner Wilhelm
Grafl Beatrice
Hammer Sabina
Lagler Julia
Liebhart Dieter
Mair Kerstin
Mitra Taniya
Pierron Alix
Schmidt Selma
Stadler Maria
Vatzia Eleni
Wernsdorf Patricia

Beteiligte Vetmed-Organisationseinheiten
Institut für Immunologie
Universitätsklinik für Geflügel und Fische, Klinische Abteilung für Geflügelmedizin


Zugehörige(s) Projekt(e): Phenotype and cytokine production of Histomonas meleagridis-specific T cells isolated from chickens and turkeys infected with the parasite or vaccinated against histomonosis


Abstract:
The protozoan parasite Histomonas meleagridis is the causative agent of the re-emerging disease histomonosis of chickens and turkeys. Due to the parasite's extracellular occurrence, a type-2 differentiation of H. meleagridis-specific T cells has been hypothesized. In contrast, a recent study suggested that IFN-γ mRNA+ cells are involved in protection against histomonosis. However, the phenotype and cytokine production profile of H. meleagridis-specific T cells still awaits elucidation. In this work, clonal cultures of a virulent monoxenic strain of H. meleagridis were used for infecting chickens to detect IFN-γ protein and IL-13 mRNA by intracellular cytokine staining and PrimeFlow™ RNA Assays, respectively, in CD4+ and CD8β+ T cells. Infection was confirmed by characteristic pathological changes in the cecum corresponding with H. meleagridis detection by immunohistochemistry and H. meleagridis-specific antibodies in serum. In splenocytes stimulated either with H. meleagridis antigen or PMA/ionomycin, IFN-γ-producing CD4+ T cells from infected chickens increased in comparison to cells from non-infected birds 2 weeks and 5 weeks post-infection. Additionally, an increase of IFN-γ-producing CD4-CD8β- cells upon H. meleagridis antigen and PMA/ionomycin stimulation was detected. Contrariwise, frequencies of IL-13 mRNA-expressing cells were low even after PMA/ionomycin stimulation and mainly had a CD4-CD8β- phenotype. No clear increase of IL-13+ cells related to H. meleagridis infection could be found. In summary, these data suggest that H. meleagridis infection induces a type-1 differentiation of CD4+ T cells but also of non-CD4+ cells. This phenotype could include γδ T cells, which will be addressed in future studies.

Keywords Pubmed: Animals
Chickens
Cytokinesimmunology
Phenotype
Poultry Diseasesimmunologyparasitology
Protozoan Infections, Animalimmunologyparasitology
T-Lymphocytesimmunology
Trichomonadidaphysiology

© Veterinärmedizinische Universität Wien Hilfe und Downloads