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Gewählte Publikation:

[Article in Press]

Publikationstyp: Zeitschriftenaufsatz
Dokumentart: Originalarbeit

Publikationsjahr: 2020

AutorInnen: Ullrich, E; Heidinger, P; Soh, J; Villanova, L; Grabuschnig, S; Bachler, T; Hirschböck, E; Sánchez-Heredero, S; Ford, B; Sensen, M; Rosales Rodriguez, I; Schwendenwein, D; Neumeister, P; Zurl, CJ; Krause, R; Khol, JL; Sensen, CW

Titel: Evaluation of Host-based Molecular Markers for the Early Detection of Human Sepsis.

Quelle: J Biotechnol. 2020;



Autor/innen der Vetmeduni Vienna:

Khol Johannes

Beteiligte Vetmed-Organisationseinheiten
Universitätsklinik für Wiederkäuer, Klinische Abteilung für Wiederkäuermedizin


Abstract:
We have identified 24 molecular markers, based on circulating nucleic acids (CNA) originating from the human genome, which in combination can be used in a quantitative real-time PCR (qPCR) assay to identify the presence of human sepsis, starting two to three days before the first clinical signs develop and including patients who meet the SEPSIS-3 criteria. The accuracy was more than 87% inside of the same patient cohort for which the markers were developed and up to 81% in blind studies of patient cohorts which were not included in the marker development. As our markers are host-based, they can be used to capture bacterial as well as fungal sepsis, unlike the current PCR-based tests, which require species-specific primer sets for each organism causing human sepsis. Our assay directly uses an aliquot of cell-free blood as the substrate for the PCR reaction, thus allowing to obtain the diagnostic results in three to four hours after the collection of the blood samples.


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