Ullrich, E; Heidinger, P; Soh, J; Villanova, L; Grabuschnig, S; Bachler, T; Hirschböck, E; Sánchez-Heredero, S; Ford, B; Sensen, M; Rosales Rodriguez, I; Schwendenwein, D; Neumeister, P; Zurl, CJ; Krause, R; Lorenz Khol, J; Sensen, CW
Evaluation of Host-based Molecular Markers for the Early Detection of Human Sepsis.
J Biotechnol. 2020; 310:80-88
Autor/innen der Vetmeduni Vienna:
Universitätsklinik für Wiederkäuer, Klinische Abteilung für Wiederkäuermedizin
- We have identified 24 molecular markers, based on circulating nucleic acids (CNA) originating from the human genome, which in combination can be used in a quantitative real-time PCR (qPCR) assay to identify the presence of human sepsis, starting two to three days before the first clinical signs develop and including patients who meet the SEPSIS-3 criteria. The accuracy was more than 87 % inside of the same patient cohort for which the markers were developed and up to 81 % in blind studies of patient cohorts which were not included in the marker development. As our markers are host-based, they can be used to capture bacterial as well as fungal sepsis, unlike the current PCR-based tests, which require species-specific primer sets for each organism causing human sepsis. Our assay directly uses an aliquot of cell-free blood as the substrate for the PCR reaction, thus allowing to obtain the diagnostic results in three to four hours after the collection of the blood samples.Copyright © 2020 Elsevier B.V. All rights reserved.