Background: Hepatic lipidosis is a common condition in replies but is rarely reported in amphibians. Case description: A 1-year-old tiger salamander (Ambystoma tigrinum) of unknown sex, was presented with a 6-week history of anorexia. Diagnostic tests performed included an in-house hematological evaluation, blood biochemistry, radiology, coelomic ultrasound, and computed tomography (CT), which revealed hepatic lipidosis. Hospitalization for supportive medical management included enteral nutrition, antibiotic, and liver support medication. Conclusions and case relevance: The salamander responded well to medical management and started eating a week after discharge. To the authors' knowledge, this is the first report of diagnosis of hepatic lipidosis in a salamander by quantitative CT, which represents a noninvasive alternative to liver biopsy.