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Gewählte Publikation:

Publikationstyp: Zeitschriftenaufsatz
Dokumenttyp: Originalarbeit

Jahr: 2005

AutorInnen: Chvala, S; Bakonyi, T; Hackl, R; Hess, M; Nowotny, N; Weissenböck, H

Titel: Limited pathogenicity of Usutu virus for the domestic chicken (Gallus domesticus).

Quelle: Avian Pathol. 2005; 34(5):392-395



Autor/innen der Vetmeduni Vienna:

Bakonyi Tamas
Chvala-Mannsberger Sonja
Hess Michael
Nowotny Norbert
Weissenböck Herbert

Beteiligte Vetmed-Organisationseinheiten
Institut für Pathologie
Institut für Virologie
Universitätsklinik für Geflügel und Fische


Zugehörige(s) Projekt(e): Auftreten von Usutu Virus in Österreich: Erforschung des Virus, Etablierung von Nachweismethoden, Auswirkung der Infektion auf Mensch, Haus- und Wildtiere


Abstract:
In summer 2001, Usutu virus (USUV), a mosquito-borne flavivirus, was isolated for the first time in Europe during a mortality incident among Eurasian blackbirds (Turdus merula) in Austria. Chickens are frequently used as sentinel animals for arbovirus surveillance systems. In the present study, the pathogenicity of USUV for specific pathogen free chickens was investigated. Ten 2-week-old chickens were inoculated intravenously with 0.1 ml inoculum containing 10(3) median (50%) tissue culture infectious dose of USUV strain Vienna 2001-blackbird (939/01). Clinical signs, viraemia, gross and microscopic lesions, contact transmission and immunological response were evaluated. No clinical signs were observed in the USUV-inoculated animals during the experimental period. Pathological examination showed moderate splenomegaly and follicular infiltrates in the liver of several inoculated animals. Mild non-suppurative encephalitis was observed in the brain tissue of one virus-inoculated chicken examined 7 days post inoculation (d.p.i.). USUV nucleic acid was detected by reverse-transcriptase polymerase chain reaction in the organs of six inoculated chickens, although immunohistochemistry for flavivirus antigen was negative in all tissues from all chickens. Virus shedding was shown in three inoculated birds by detecting USUV RNA in cloacal swabs of two chickens at 5 d.p.i., and in the cloacal and pharyngeal swabs of one chicken at 7 d.p.i. Based on detection of viral RNA in peripheral blood mononuclear cells, viraemia was detected only in two chickens (at 7 d.p.i.). Only one of the inoculated chickens developed an antibody response. There was no evidence of virus transmission to chickens kept in contact with inoculated birds. No USUV was isolated from in-contact birds and all in-contact and control animals lacked USUV-specific antibodies. The present data suggest that domestic chickens are not at risk of developing clinical disease following USUV infection and that chickens are unlikely to be useful for sentinel purposes in USUV surveillance programmes.

Keywords Pubmed: Animals
Brain/pathology
Chickens*
Flavivirus/genetics
Flavivirus/pathogenicity*
Flavivirus Infections/immunology
Flavivirus Infections/pathology
Flavivirus Infections/veterinary*
Immunohistochemistry/veterinary
Poultry Diseases/immunology
Poultry Diseases/pathology*
Poultry Diseases/virology*
Reverse Transcriptase Polymerase Chain Reaction/veterinary
Serologic Tests/veterinary
Specific Pathogen-Free Organisms
Viremia/veterinary


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