Manz, A; Allenspach, K; Kummer, S; Richter, B; Walter, I; Macho-Maschler, S; Tichy, A; Burgener, IA; Luckschander-Zeller, N
Upregulation of signal transducer and activator of transcription 3 in dogs with chronic inflammatory enteropathies.
J Vet Intern Med. 2021 35 (3) 1288-1296.
Autor/innen der Vetmeduni Vienna:
Institut für Tierzucht und Genetik, Abteilung für Molekulare Genetik
Institut für Pathologie
Universitätsklinik für Kleintiere, Klinische Abteilung für Interne Medizin Kleintiere
Plattform Bioinformatik und Biostatistik
- In inflammatory bowel disease (IBD) in humans, phosphorylated signal transducer and activator of transcription 3 (pSTAT3) is upregulated in mucosal epithelial cells and correlates with clinical severity.To investigate the expression pattern of pSTAT3 in the mucosa of dogs with chronic inflammatory enteropathy (CIE) and explore correlations between its expression and clinical and histopathological severity scoring.Twenty-eight canine CIE patients grouped into food-responsive enteropathy (FRE; 9), steroid-responsive enteropathy (SRE; 10), and protein-losing enteropathy (PLE; 9). Ten healthy beagle dogs served as controls (CO).Retrospective case control study. Immunohistochemistry was used to detect pSTAT3 in canine duodenal mucosa samples.Compared to CO, SRE (P < .001) and PLE (P < .001) dogs had significantly higher pSTAT3 expression in the villus epithelium. The SRE group had a significantly higher expression in the villus lamina propria (VLP) compared to controls (P = .009). In the crypt epithelium (CE), all CIE dogs had significantly higher pSTAT3 expression (FRE, P = .002; SRE, P = .003; PLE, P < .001) compared to CO. In the lamina propria crypt region (CLP), dogs with FRE (P = .04) and SRE (P = .03) had significantly upregulated pSTAT3 compared to controls. A positive correlation was found between canine chronic enteropathy clinical activity index (CCECAI) scoring and pSTAT3 expression for both epithelial (rho = .541; P < .001) and crypt regions (rho = .32; P = .02).pSTAT3 is upregulated in CIE in dogs, correlates with clinical severity, and may be helpful as a clinical marker in dogs with CIE.© 2021 The Authors. Journal of Veterinary Internal Medicine published by Wiley Periodicals LLC on behalf of American College of Veterinary Internal Medicine.
Inflammatory Bowel Diseasesveterinary
STAT3 Transcription Factor