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Gewählte Publikation:

Publikationstyp: Zeitschriftenaufsatz
Dokumenttyp: Originalarbeit

Jahr: 2011

AutorInnen: Bianchini, R; Bistoni, O; Alunno, A; Petrillo, MG; Ronchetti, S; Sportoletti, P; Bocci, EB; Nocentini, G; Gerli, R; Riccardi, C

Titel: CD4(+) CD25(low) GITR(+) cells: a novel human CD4(+) T-cell population with regulatory activity.

Quelle: Eur J Immunol. 2011; 41(8):2269-78



Autor/innen der Vetmeduni Vienna:

Bianchini Rodolfo

Diese Publikation wurde nicht im Namen der Vetmeduni Vienna erstellt und ist deshalb ausschließlich der persönlichen Publikationsliste des/der Autors/Autorin zugeordnet!


Abstract:
Treg subsets play a role in sustaining peripheral tolerance, are characterized by markers such as forkhead winged-helix transcription factor (FOXP3) and CD25, and produce suppressive cytokines, such as IL-10 and TGF-β. Glucocorticoid-induced TNF receptor family-related (GITR) protein has been suggested to regulate Treg activity in mice. The aim of our study was to investigate GITR expression in human CD4(+) T lymphocytes and its possible role in Treg function. Results indicate that a subset of CD4(+) T cells in the peripheral blood expresses GITR and low levels of CD25 (CD4(+) CD25(low) GITR(+) ). These cells show Treg features as they express FOXP3, IL-10, TGF-β and are anergic but, as opposed to natural Tregs, express low levels of CTLA-4 and are CD127(high) . CD4(+) CD25(low) GITR(+) cells represent a low percentage of the CD4(+) T-cell population (0.32-1.74%) and are mostly memory cells. Functional experiments demonstrated that CD4(+) CD25(low) GITR(+) cells have relevant suppressive activity that depends on TGF-β. Moreover, an anti-GITR Ab inhibited their suppressive activity, as observed in CD4(+) CD25(+) murine Tregs. Taken together, these data indicate that human CD4(+) CD25(low) GITR(+) cells represent a distinct Treg subpopulation.Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Keywords Pubmed: Adult
Animals
Antigens, CDimmunologymetabolism
CD4-Positive T-Lymphocytesimmunologymetabolism
CTLA-4 Antigen
Cell Proliferation
Flow Cytometry
Gene Expression
Glucocorticoid-Induced TNFR-Related Proteinimmunologymetabolism
Humans
Immunologic Memoryimmunology
Immunophenotyping
Interleukin-10immunologymetabolism
Interleukin-2 Receptor alpha Subunitimmunologymetabolism
Mice
Middle Aged
Reverse Transcriptase Polymerase Chain Reaction
T-Lymphocyte Subsetsimmunologymetabolism
T-Lymphocytes, Regulatoryimmunologymetabolism
Transforming Growth Factor betaimmunologymetabolism
Young Adult

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