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Gewählte Publikation:

Publikationstyp: Zeitschriftenaufsatz
Dokumenttyp: Originalarbeit

Jahr: 2013

AutorInnen: Alunno, A; Petrillo, MG; Nocentini, G; Bistoni, O; Bartoloni, E; Caterbi, S; Bianchini, R; Baldini, C; Nicoletti, I; Riccardi, C; Gerli, R

Titel: Characterization of a new regulatory CD4+ T cell subset in primary Sjögren's syndrome.

Quelle: Rheumatology (Oxford). 2013; 52(8):1387-96



Autor/innen der Vetmeduni Vienna:

Bianchini Rodolfo

Diese Publikation wurde nicht im Namen der Vetmeduni Vienna erstellt und ist deshalb ausschließlich der persönlichen Publikationsliste des/der Autors/Autorin zugeordnet!


Abstract:
CD4(+)CD25(low)GITR(+) T lymphocytes expressing FoxP3 and showing regulatory function have been recently described in healthy donors (HD). The objective of the study was to investigate their presence and role in patients with primary SS (pSS).CD4(+)CD25(low)GITR(+) cells circulating in peripheral blood (PB) of patients with pSS were isolated by MACS technique, their phenotype was studied by flow cytometry and real-time PCR, and their function was studied by in vitro co-culture. CD4(+)CD25(low)GITR(+) cells infiltrating salivary glands (SGs) were revealed by immunohistochemistry.Results indicated that conventional CD4(+)CD25(high) regulatory T cells (Tregs) are decreased, whereas CD4(+)CD25(low)GITR(+) cells are expanded in the PB of pSS as compared with HD. Phenotypic analysis demonstrated that CD4(+)CD25(low)GITR(+) cells display Treg markers, including FoxP3, TGF-β and IL-10, and functional experiments demonstrated that they exert a strong inhibitory activity against autologous effector cells. CD4(+)CD25(low)GITR(+) cells were detectable in great number in the SG inflammatory infiltrate. Interestingly, PB CD4(+)CD25(low)GITR(+) cell expansion was evident only in patients with inactive disease, while conventional CD4(+)CD25(high) Treg number was not associated with disease activity.The present data demonstrate that circulating CD4(+) cells expressing GITR, but with low levels of CD25 (CD4(+)CD25(low)GITR(+)), are detectable in pSS patients. These cells, displaying Treg phenotype and function, are present in SG inflamed tissues and are expanded in the PB of subjects with inactive disease. Data suggest that the expansion of CD4(+)CD25(low)GITR(+) cells in pSS may represent a counter-regulatory attempt against autoimmune-driven inflammation and may provide a new target for future treatment strategies.

Keywords Pubmed: Adult
CD4-Positive T-Lymphocytesimmunologymetabolism
CD8-Positive T-Lymphocytesimmunologymetabolism
Case-Control Studies
Cell Proliferation
Cells, Cultured
Disease Progression
Female
Flow Cytometry
Fluorescent Antibody Technique
Humans
Immunohistochemistry
Logistic Models
Male
Middle Aged
Real-Time Polymerase Chain Reaction
Reference Values
Sjogren's Syndromeimmunologypathologyphysiopathology
Statistics, Nonparametric
T-Lymphocyte Subsetsimmunologymetabolism
T-Lymphocytes, Regulatoryimmunology

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