Veterinärmedizinische Universität Wien Forschungsinformationssystem VetDoc

Grafischer Link zur Startseite der Vetmeduni Vienna

Gewählte Publikation:

Publikationstyp: Zeitschriftenaufsatz
Dokumenttyp: Originalarbeit

Jahr: 2017

AutorInnen: Di Benedetto, G; Saccone, S; Lempereur, L; Ronsisvalle, N; Nocentini, G; Bianchini, R; Riccardi, C; Bernardini, R; Cantarella, G

Titel: The Proinflammatory Cytokine GITRL Contributes to TRAIL-mediated Neurotoxicity in the HCN-2 Human Neuronal Cell Line.

Quelle: Curr Alzheimer Res. 2017; 14(10):1090-1101



Autor/innen der Vetmeduni Vienna:

Bianchini Rodolfo

Diese Publikation wurde nicht im Namen der Vetmeduni Vienna erstellt und ist deshalb ausschließlich der persönlichen Publikationsliste des/der Autors/Autorin zugeordnet!


Abstract:
Cytokines belonging to the TNF superfamily play a relevant role in neurodegenerative processes. Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand (TRAIL), released during neuronal injury, has proven to potently mediate and sustain neurotoxic processes leading to neuronal death. Similarly to TRAIL, the cytokine Glucocorticoid-induced TNF receptor ligand (GITRL) is able to transduce proapoptotic signals. In spite of the array of reports suggesting relationships between TRAIL and other cytokines, scanty data are, so far, available about a GITRL/TRAIL crosstalk.Here, we investigated possible interactions between TRAIL and the GITRL system in an in vitro model of neurodegeneration, using the human cortical neuronal cell line HCN-2. Cultured HCN-2 neurons were incubated at different times with GITRL and/or TRAIL, and thereafter nucleic acid and protein expression were measured. Real-time PCR analysis showed that the human cortical neuronal cell line HCN-2 does not express GITRL mRNA, but the latter is induced after treatment with TRAIL. In addition, HCN-2 cells did not express the GITRL receptor GITR mRNA, neither in control cultures, nor after treatment with TRAIL. All mRNA data were confirmed by western blot analysis of proteins. Cell viability assay showed that TRAIL, when associated to GITRL, was able to exert additive toxic effects. A counterproof was provided in experiments performed blocking GITRL, in which TRAIL-mediated toxicity appeared significantly reduced. Results suggest that GITRL/TRAIL redundancy during neurodegenerative processes implies extended potentiation of detrimental effects of both cytokines on neurons, eventually leading to larger cell damage and death.Finally, characterization of novel molecular targets within the TRAIL/GITRL interplay may represent a platform for innovative therapy of neurodegenerative disorders.Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.org.

Keywords Pubmed: Apoptosisgeneticsphysiology
Caspasesmetabolism
Cell Line
Cell Survivalphysiology
Cerebral Corteximmunologypathology
Gene Expression
Glucocorticoid-Induced TNFR-Related Proteinmetabolism
Humans
Neuroimmunomodulationphysiology
Neuronsimmunologypathology
Phosphorylation
RNA, Messengermetabolism
Receptors, TNF-Related Apoptosis-Inducing Ligandmetabolism
STAT3 Transcription Factormetabolism
TNF-Related Apoptosis-Inducing Ligandmetabolismtoxicity
Tumor Necrosis Factor Inhibitors
Tumor Necrosis Factorsadministration & dosagemetabolism

© Veterinärmedizinische Universität Wien Hilfe und DownloadsErklärung zur Barrierefreiheit