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Selected Publication:

Publication type: Journal Article
Document type: Note

Year: 2015

Author(s): Steinberger, B; Brem, G; Mayrhofer, C

Title: Evaluation of SYPRO Ruby total protein stain for the normalization of two-dimensional Western blots.

Source: Anal Biochem. 2015; 476:17-19



Authors Vetmeduni Vienna:

Brem Gottfried,
Itze-Mayrhofer Corina,
Steinberger Birgit,

Vetmed Research Units
Unit of Reproductive Biology,


Abstract:
Due to post-translational modifications such as phosphorylation, proteins exist as distinct charge variants. Two-dimensional (2D) gel electrophoresis followed by immunoblotting enables the detection of these isoforms. For their accurate relative quantitation in different samples, a loading control is necessary to compensate for technical errors such as imprecise sample loading or transfer. The study reveals that the combinatory approach of SYPRO Ruby and chemiluminescence-based 2D Western blot analysis exhibits high linearity and excellent reproducibility and is applicable for limited sample amounts.

Keywords Pubmed: Blotting, Western
Electrophoresis, Gel, Two-Dimensional/methods*
Organometallic Compounds/chemistry*


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