University of Veterinary Medicine Vienna - Research portal

Diagrammed Link to Homepage University of Veterinary Medicine, Vienna

Selected Publication:

Publication type: Baccalaureate Thesis

Year: 2015

Author(s): Fischer, Kristin

Title: Untersuchung der genetischen Diversität eurasischer Populationen des Wildschweins (Sus scrofa).

Other title: Analysis of the genetic diversity of Eurasian wild boar populations (Sus scrofa): Variability of the mitochondrial DNA

Source: Bakkalaureatsarbeit, Vet. Med. Univ. Wien, pp. 25.


Advisor(s):

Brem Gottfried

Reviewer(s):
Burger Pamela

Vetmed Research Units:
Unit of Reproductive Biology


Abstract:
The population of the wild boar, Sus Scrofa, can be divided into two big groups, the European and the Asian one. In this study their geographic distribution in Eurasia was investigated. For that purpose, Russia was subdivided into three regions. The arrangement was classified by its federation parts, Ural and the Far East in addition to „European Russia“. The term „European Russia“ includes the federation parts northwest Russia, central Russia, Volga, south Russia and north Caucasus. The received tissue- and DNA samples, derived from 75 wild boars with known geographical origin in Russia, were analyzed for their D-loop region of the mtDNA and thereby determining its maternal origin. The D-loop region contains high amounts of mutations and therefore is useful to investigate the phylogenetical background of closely related populations. The haplogenetic classification was performed via comparison to reference sequences of European (Giuffra, Kijas et al. 2000) and Asian (Ramayo, Shemeret’eva et al. 2011) wild boars. As an outgroup the columbian collared peccary (Tayassu tajacu) was utilized (Kim, Lee et al. 2002). To conduct a chi-square-test, three haplotypes had to be defined. The corresponding comparison of frequencies between the wild boar populations of the European part of Russia and the Ural region was not significant (p=1). However, there was a significant difference (p=0.0005) between the European part of Russia and the Far East. Thus, the European part of Russia and the Ural region are therefore closely related, whereas the haploypes in the Far East differ significantly from the other regions. The illustration of the sequences in a phylogenetic network confirmed these results. The eastwest border of the populations could only be determined approximately to Siberia. The European wild boars are mainly westwards of Siberia, while the Asian ones are mainly in the east. The Ural, which reaches from north to the south of Russia, is a part of the european-asian border, but does not act as a natural barrier for the genetical distribution of wild boars.


© University of Veterinary Medicine ViennaHelp and Downloads