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Selected Publication:

Type of publication: Journal Article
Type of document: Full Paper

Year: 2011

Authors: Mader, E; Ruzicka, J; Schmiderer, C; Novak, J

Title: Quantitative high-resolution melting analysis for detecting adulterations.

Source: Anal Biochem. 2011; 409(1):153-155

Authors Vetmeduni Vienna:

Mader Eduard
Novak Johannes
Ruzicka Joana
Schmiderer Corinna

Vetmed Research Units
Institute of Animal Nutrition and Functional Plant Compounds

Admixtures of different plant species are a common problem in raw materials for medicinal use. Two exemplary assays were developed to admixtures in Helleborus niger with high-resolution melting analysis. HRM proved to be a very sensitive tool in detecting admixtures, able to detect a ratio of 1:1000 with unknown species, and of 1:200,000 with Veratrum nigrum. The example proves the ability of HRM for quantification in multiplex PCR. The method is not limited to detecting adulterations. It can also be used to quantify a specific target by integrating a second amplicon in the assay as internal standard.

Keywords Pubmed: DNA Primers/chemistry
DNA, Plant/chemistry*
Nucleic Acid Denaturation
Polymerase Chain Reaction/methods*
Spectrometry, Fluorescence
Transition Temperature

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