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Type of publication: Journal Article
Type of document: Full Paper

Year: 2011

Authors: Yunus, AW; Böhm, J

Title: A simple method for producing Aflatoxin B(1) on rice medium for use in experimental animal feeds.

Source: J Anim Plant Sci (21), 2 303-304.

Authors Vetmeduni Vienna:

Böhm Josef

Vetmed Research Units
Institute of Animal Nutrition and Functional Plant Compounds

A simplification and standardisation of the existing methods to produce aflatoxin B-1 (AFB(1)) on rice medium is described. As per conventional method, rice were first soaked in 1 ml water/2 g of rice for 2 hr, autoclaved, and then inoculated with Aspergillus flavus. During different stages of incubation, water was added according to different regimens. It was concluded that 1 ml water/100 g of rice at 24 to 36 hr of incubation is the maximum amount of water that could be added for optimal AFB(1) production.

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