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Publication type: Journal Article
Document type: Full Paper

Year: 2013

Author(s): G√ľnes, A; Marek, A; Hess, M

Title: Species determination of fowl adenoviruses based on the 52K gene region.

Source: Avian Dis. 2013; 57(2):290-294

Authors Vetmeduni Vienna:

Hess Michael
Marek Ana

Vetmed Research Units
University Clinic for Poultry and Fish Medicine, Clinical Unit of Poultry Medicine

In the present study, the classification of fowl adenoviruses (FAdVs) based on a part of the 52K gene region was described. A total of 44 FAdV field samples from different countries and sources were detected using a recently developed SYBR Green-based real-time PCR. Amplified products were sequenced, and phylogenetic analyses were conducted on the basis of the 116-bp region. For comparison, the already published sequences of the 52K gene region of aviadenoviruses were used in the analyses. The phylogenetic analysis allowed the grouping of the FAdVs into the established five different FAdV species: Fowl adenovirus A to Fowl adenovirus E. The existence of the species was supported by high bootstrap values (> 70%). This method provides the advantages of quantitation and high sensitivity for FAdV detection in combination with species assignment.

Keywords Pubmed: Adenoviridae Infections/diagnosis
Adenoviridae Infections/veterinary*
Adenoviridae Infections/virology
Amino Acid Sequence
Fowl adenovirus A/classification
Fowl adenovirus A/genetics
Fowl adenovirus A/metabolism
Genome, Viral*
Molecular Sequence Data
Poultry Diseases/diagnosis
Poultry Diseases/virology*
Real-Time Polymerase Chain Reaction/methods*
Real-Time Polymerase Chain Reaction/veterinary
Sequence Alignment/veterinary
Sequence Analysis, DNA/veterinary
Viral Proteins/chemistry
Viral Proteins/genetics
Viral Proteins/metabolism

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