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Selected Publication:

Type of publication: Journal Article
Type of document: Full Paper

Year: 2014

Authors: Schybli, M; Sigrist, B; Hess, M; van Leerdam, B; Hoop, RK; Vögtlin, A

Title: Development of a new real-time polymerase chain reaction assay to detect Duck adenovirus A DNA and application to samples from Swiss poultry flocks.

Source: J Vet Diagn Invest. 2014; 26(2):189-194

Authors Vetmeduni Vienna:

Hess Michael

Vetmed Research Units
University Clinic for Poultry and Fish Medicine, Clinical Unit of Poultry Medicine

Between 2008 and 2012, commercial Swiss layer and layer breeder flocks experiencing problems in laying performance were sampled and tested for infection with Duck adenovirus A (DAdV-A; previously known as Egg drop syndrome 1976 virus). Organ samples from birds sent for necropsy as well as blood samples from living animals originating from the same flocks were analyzed. To detect virus-specific DNA, a newly developed quantitative real-time polymerase chain reaction method was applied, and the presence of antibodies against DAdV-A was tested using a commercially available enzyme-linked immunosorbent assay. In 5 out of 7 investigated flocks, viral DNA was detected in tissues. In addition, antibodies against DAdV-A were detected in all of the flocks.

Keywords Pubmed: Adenoviridae Infections/diagnosis
Adenoviridae Infections/epidemiology
Adenoviridae Infections/veterinary*
Antibodies, Viral/blood
Atadenovirus/isolation & purification*
DNA, Viral/isolation & purification
Enzyme-Linked Immunosorbent Assay/veterinary
Poultry Diseases/diagnosis
Poultry Diseases/epidemiology
Poultry Diseases/virology*
Real-Time Polymerase Chain Reaction/veterinary*
Sensitivity and Specificity
Specific Pathogen-Free Organisms

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