In this cross-over study 19 European Short-hair cats - 10 adult and 9 old ones - were fed with an n-3 enriched diet (n-6:n-3 = 0,4:1) and a control diet (n-6:n-3 = 7,2:1) for eight weeks each. Blood was collected on day 0, 28, 56, 84 and 112 via jugular venipuncture and tested haematologically and chemically (plasma triglyceride level, liver parameters, bile acids, cholesterol, HDL-cholesterol). The coagulation parameters and fatty acid pattern were also evaluated. For showing the fatty acid pattern, total lipid was collected from plasma with a modified Folch method and then separated with gas chromatography. As expected the plasma fatty acid pattern showed significant differences between the treatments. The n-6:n-3 relation in plasma of cats fed the test diet was 1,3:1, of those that were given control diet 8,2:1.
Plasma triglyceride level was significant lower (0,21 mmol/l) in cats supplemented with fish oil than in those fed the chicken fat diet (0,30 mmol/l). There occurred also differences between first (0,29 mmol/l) and second period (0,22 mmol/l). Cholesterol (15 %) and HDL-cholesterol (14 %) also decreased in the test group. There was no difference in Cholesterol/HDL-Cholesterol quotient. Alkaline phosphatase was significantly lower by treatment with n-3 enriched diet (20 %).
During feeding the test diet the number of eosinophile granulocytes increased. The level was twice as high as in the control group.
Coagulation parameters increased slightly during the study. Significant differences in relation with the diet could not be found.
cat fish oil triglyceride level / cholesterol alkaline phosphatase