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Publication type: Diploma Thesis

Year: 2011

Author(s): Braunsberger, SV

Title: Nachweis von Phytosterolen in Nahrungsergänzungsmitteln und einem Futterergänzungsmittel mit Extrakten von Cucurbita pepo var. styriaca mittels Dünnschichtchromatographie und SPME-Gaschromatographie/Massenspektrometrie.

Other title: Verification of phytosterols in nutritional supplements containing extracts of Cucurbita pepo var. styriaca by use of thin layer chromatography and SPME-gas chromatography/mass spectrometry

Source: Diplomarbeit, Vet. Med. Univ. Wien, pp. 60.


Advisor(s):

Zitterl-Eglseer Karin

Reviewer(s):
Möstl Erich

Vetmed Research Units:
Institute of Animal Nutrition and Functional Plant Compounds


Graduation date: 25.09.12


Abstract:
The aim of this work was to analyze phytosterols in nutritional supplements for humans and animals containing pumpkin seed extracts with thin-layerchromatography for qualitative analyses and to test the practicability of a SPME-GC/MS method of determining phytosterols in blood serum. The thin-layer-chromatographic analysis followed the standard operation procedure of the DC-Atlas (PACHALY 2010). The nutritional supplements named Klosterfrau Kürbisplus, Aurita Kürbiskern Plus, Mondosan Bio Kürbiskern Plus und Magister Doskar Kürbiskern showed the best spectra compared to the DC-Atlas (PACHALY 2010). The gas-chromatographic analysis was conducted according to a method of DOMENO et al. published in the year 2005. This used method was quite costly and time-consuming. A price comparison of the nutritional products was made as well. Herbal nutritional supplements are a very good alternative to chemical drugs, and also to phytopharmaceuticals especially because of their low or even nonexistent side-effects and simplified marketing authorisation processes. Nutritional supplements containing pumpkin seed extracts are mainly used as treatment against benign prostatic hypertrophy.


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