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Type of publication: Diploma Thesis
Type of document:

Year: 2012

Authors: Stejskal, Manuela

Title: Untersuchung der Auswirkung von Procox® auf die Darmflora von Welpen.

Other title: Examination of the effect on the gut flora of puppies by procox®

Source: Diplomarbeit, Vet. Med. Univ. Wien, pp. 56.


Ehling-Schulz Monika

Iben Christine

Vetmed Research Units:
Institute of Microbiology, Functional Microbiology Division

Graduation date: 24.09.12

The aim of the present study was to examine the effect of antiparasitic drug Procox® on the increase and colonization of pathogen microbes of puppies in their first weeks of life by bacteriological examinations. Therefore six litters containing different breeds with a total of 46 puppies were taken. Within litters, puppies were randomly split into a Procox® group and a control group. The Procox® groups (n = 24) received 0,5 ml Procox® per kg body weight, corresponding to 0,45mg emodepside and 9 mg toltrazuril per kg body weight in the 3rd, 5th and 7th week of life. The control groups (n = 22) were treated with conventional antiparasitic drugs. 15 puppies got Banminth® (5 mg pyrantel base per kg body weight) and 7 puppies got Drontal® (5 mg praziquantel and 57,5 mg pyrantelembonate per kg body weight) at the same time. All probands were examined clinically every day from 3rd till 8th week of life and were weighted. Samples of faeces for bacteriological examination were taken from every puppy of litter 1, 2 and 3 in the 4th, 6th and 8th week of life. In litter 4, 5 and 6 samples of faeces of every puppy were taken only in the 8th week of life. The pathogen microbes hemolytic Escherichia coli, Clostridium perfringens, Salmonella spp. and Candida albicans were used to detect changes in the gut flora. At the clinical examination there were neither abnormalities nor changes in faeces consistency. Procox® groups and control groups of litter 1, 2 and 3 were compared in the 4th, 6th and 8th week of life. No significant difference existed between Procox® groups and control groups (p-values of hemolytic E. coli: 4th week of life p = 0,923, 6th week of life p = 0,539, 8th week of life p = 0,381. p-values of C. perfringens: 4th week of life p = 0,346, 6th week of life p = 0,497, 8th week of life p = 0,418. p-values of C. albicans: 4th week of life p = 0,974, 6th week of life p = 1, 8th week of life p = 1). Also the comparison of Procox® groups and control groups of all six litters in the 8th week of life provided no significant difference (hemolytic E. coli: p = 0,236, C. perfringens: p = 0,406, C. albicans: p = 0,600). The low number of probands which were taken and the fact that factors of influence on the gut flora like nutrition, environment and immunity which were not considered, could be causes of these results. Data on possible effects by antiparasitic drugs on the canine gut microbiota are currently not available. The present study showed that there is no evidence for significant effect of Procox® on canine gut flora. Hence, from a bacteriological point of view there are no objections to use Procox® for puppies.

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