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Publication type: Journal Article
Document type: Full Paper

Year: 2008

Author(s): Kanca, H; Walter, I; Schäfer-Somi, S; Budik, S; Ay, SS; Kucukaslan, I; Agaoglu, AR; Izgur, H; Aslan, S

Title: Induction of abortion with aglepristone significantly changed the expression of progesterone and estrogen receptors in canine endometrial stromal cells.

Source: Theriogenology. 2008; 70(9):1439-1448

Authors Vetmeduni Vienna:

Budik Sven
Schäfer-Somi Sabine
Walter Ingrid

Vetmed Research Units
Insemination and Embryotransfer Platform
Institute of Topographical Anatomy

In the present study, resorption/abortion was induced between days 25 and 45 of gestation with aglepristone (group IRA, n=10). The aim was to observe the change in the distribution of progesterone (PR) and estrogen receptors (ER), in comparison to a group of spontaneous resorptions/abortions (group SRA, n=5), and a further group of normal healthy pregnant animals, ovariohysterectomized between days 25 and 45 of gestation (controls, n=7). The receptors were assessed by means of immunohistochemistry (IHC) and RT-PCR, at the placental and interplacental sites of the uterine horn as well as in the corpus uteri. Significant differences were observed between the controls on one side and the groups of resorption/abortion on the other side. The total scores of the progesterone receptors (TPR) in the placental and interplacental part of the uterine horn, was significantly lower in the endometrial stromal cells (ESC) of the control group than in those of the SRA- and IRA-group, respectively (placenta: 5.8 vs. 6.5 and 6.7, p<0.01; interplacental sites: 5.6 vs. 6.6 and 6.6, p<0.05). In contrary, the total scores of the estrogen receptors (TER) at interplacental sites and the corpus uteri, respectively, was significantly higher in the myometrial smooth muscle cells (MSMC) and the ESC (p<0.05) of the controls. We therefore conclude, that the here observed differences between groups point to an up-regulation of TPR- and a down-regulation of TER-scores in endometrial stromal cells at different uterine sites during resorption/abortion, which indicates a special role of these cells.

Keywords Pubmed: Abortifacient Agents/pharmacology
Abortion, Induced/veterinary*
Abortion, Veterinary/chemically induced*
Gene Expression Regulation/drug effects
Receptors, Estrogen/genetics
Receptors, Estrogen/metabolism*
Receptors, Progesterone/genetics
Receptors, Progesterone/metabolism*
Reverse Transcriptase Polymerase Chain Reaction/veterinary
Stromal Cells/cytology
Stromal Cells/metabolism

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