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Publication type: Journal Article
Document type: Full Paper

Year: 2012

Author(s): Hoelker, M; Salilew-Wondim, D; Drillich, M; Christine, GB; Ghanem, N; Goetze, L; Tesfaye, D; Schellander, K; Heuwieser, W

Title: Transcriptional response of the bovine endometrium and embryo to endometrial polymorphonuclear neutrophil infiltration as an indicator of subclinical inflammation of the uterine environment.

Source: Reprod Fertil Dev. 2012; 24(6):778-793



Authors Vetmeduni Vienna:

Drillich Marc


Abstract:
The aim of the present study was to analyse the effect of subclinical endometritis on endometrial and embryonic gene expression. A total of 49 cows at either Day 0 or Day 7 of the oestrous cycle (62-83 days post partum) following superovulation were classified as having subclinical endometritis (SE-0, SE-7) or a healthy endometrium (HE-0, HE-7) on the basis of endometrial cytological evaluation. Endometrial samples and associated embryos were subjected to global transcriptome analysis using the Bovine GeneChip (Affymetrix, Santa Clara, CA, USA) and aberrant transcript profiles were observed in SE-0 and SE-7 cows. At Day 0, 10 transcripts were found to be differentially expressed in endometrial samples. Specifically, the PDZK1, PXDN, DDHD2, GPLD1 and SULT1B1 genes were downregulated, whereas the PKIB, LOC534256, BT29392, LYZ and S100A14 genes were upregulated in SE-0 cows. Similarly, 11 transcripts were found to be differentially regulated on Day 7. Of these, GNPTG, BOLA-DQA5, CHD2, LOC541226, VCAM1 and ARHGEF2 were found to be downregulated, whereas PSTPIP2, BT236441 and MGC166084 were upregulated in SE-7 cows. Accordingly, endometrial health status affected the number of flushed, transferable embryos. In all, 20 genes were differentially regulated in blastocysts derived from HE-7 and SE-7 cows. Of these, GZMK, TCEAL4, MYL7, ADD3 and THEM50B were upregulated, whereas NUDCD2, MYO1E, BZW1, EHD4 and GZMB were downregulated. In conclusion, endometrial polymorphonuclear neutrophil infiltration as an indicator of subclinical endometritis is associated with changes in endometrial gene expression patterns, including genes involved in cell adhesion and immune modulation. Consequently, subclinical endometritis affects gene expression in embryos, including the expression of genes related to membrane stability, the cell cycle and apoptosis.

Keywords Pubmed: Animals
Blastocyst/immunology
Blastocyst/metabolism*
Cattle
Cattle Diseases/genetics*
Cattle Diseases/immunology
Cattle Diseases/pathology
Dairying
Endometritis/genetics
Endometritis/immunology
Endometritis/pathology
Endometritis/veterinary*
Endometrium/immunology
Endometrium/metabolism*
Endometrium/pathology
Estrous Cycle
Female
Gene Expression Profiling/veterinary
Gene Expression Regulation
Insemination, Artificial/veterinary
Neutrophil Infiltration/genetics*
Pregnancy
Puerperal Disorders/genetics
Puerperal Disorders/immunology
Puerperal Disorders/pathology
Puerperal Disorders/veterinary*
RNA, Messenger/metabolism
Superovulation
Time Factors
Transcription, Genetic*


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