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Selected Publication:

Type of publication: Journal Article
Type of document: Full Paper

Year: 2013

Authors: Strasser, A; Carra, M; Ghareeb, K; Awad, W; Böhm, J

Title: Protective effects of antioxidants on deoxynivalenol-induced damage in murine lymphoma cells.

Source: Mycotoxin Res. 2013; 29(3):203-208



Authors Vetmeduni Vienna:

Awad Wageha
Böhm Josef
Carra Mirja
Ghareeb Khaled
Strasser Alois

Vetmed Research Units
University Clinic for Poultry and Fish Medicine, Clinical Unit of Poultry Medicine
Institute of Animal Nutrition and Functional Plant Compounds
Institute of Physiology, Pathohysiology and Biophysics, Unit of Physiology, Pathophysiology, and Experimental Endocrinology


Abstract:
As contradictory results have been reported on the immunotoxic properties of deoxynivalenol (DON) in animal studies, we introduced a lymphoblast cell culture model in order to examine the effects of DON on lymphoblastic cell growth and metabolism as well as the preventive properties of free radical scavenger molecules against the DON-induced cell damage. Murine YAC-1 lymphoma cells were used because lymphoblasts have been shown to be sensitive to DON-induced immunotoxicity. Cells were quantified and their proliferative activity was measured by a proliferation test. Lipid peroxidation and protein oxidation were determined using assays quantifying thiobarbituric acid reactive substances (TBARS) and carbonylated proteins. Severely reduced cell counts were detected in DON-treated samples, confirmed by a 5-10 times lower proliferative activity. Significant increases in lipid peroxidation and protein oxidation were found in parallel incubated samples. The pre-incubation with free radical scavengers significantly reduced DON-induced changes to proteins and lipids as well as the tarnished cell viability and cell proliferation. These results suggest that YAC-1 lymphoma cells are a suitable model to investigate and elucidate the basic molecular and cellular mechanisms for possible immunotoxic effects of DON. With regard to the impact of free radical scavengers, the applied in-vitro model might enable the investigation of potential prophylactic and therapeutic effects before or even without harmful animal experiments and cost- and time-intensive expenses.

Keywords Pubmed: Animals
Antioxidants/metabolism*
Cell Count
Cell Line, Tumor
Cell Proliferation/drug effects
Cell Survival/drug effects
Lipid Peroxidation
Lymphocytes/drug effects*
Mice
Oxidation-Reduction
Proteins/metabolism
Trichothecenes/antagonists & inhibitors*
Trichothecenes/toxicity*


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