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Selected Publication:

Type of publication: Journal Article
Type of document: Full Paper

Year: 2016

Authors: Dzieciol, M; Schornsteiner, E; Muhterem-Uyar, M; Stessl, B; Wagner, M; Schmitz-Esser, S

Title: Bacterial diversity of floor drain biofilms and drain waters in a Listeria monocytogenes contaminated food processing environment.

Source: Int J Food Microbiol. 2016; 223:33-40

Authors Vetmeduni Vienna:

Dzieciol Monika
Muhterem-Uyar Meryem
Schmitz-Esser Stephan
Schornsteiner Elisa
Stessl Beatrix
Wagner Martin

Vetmed Research Units
Institute of Food Safety, Food Technology and Veterinary Public Health, Unit of Food Microbiology

Project(s): Protection of consumers by microbial risk mitigation through combating segregation of expertise

Sanitation protocols are applied on a daily basis in food processing facilities to prevent the risk of cross-contamination with spoilage organisms. Floor drain water serves along with product-associated samples (slicer dust, brine or cheese smear) as an important hygiene indicator in monitoring Listeria monocytogenes in food processing facilities. Microbial communities of floor drains are representative for each processing area and are influenced to a large degree by food residues, liquid effluents and washing water. The microbial communities of drain water are steadily changing, whereas drain biofilms provide more stable niches. Bacterial communities of four floor drains were characterized using 16S rRNA gene pyrosequencing to better understand the composition and exchange of drain water and drain biofilm communities. Furthermore, the L. monocytogenes contamination status of each floor drain was determined by applying cultivation-independent real-time PCR quantification and cultivation-dependent detection according to ISO11290-1. Pyrosequencing of 16S rRNA genes of drain water and drain biofilm bacterial communities yielded 50,611 reads, which were clustered into 641 operational taxonomic units (OTUs), affiliated to 16 phyla dominated by Proteobacteria, Firmicutes and Bacteroidetes. The most abundant OTUs represented either product- (Lactococcus lactis) or fermentation- and food spoilage-associated phylotypes (Pseudomonas mucidolens, Pseudomonas fragi, Leuconostoc citreum, and Acetobacter tropicalis). The microbial communities in DW and DB samples were distinct in each sample type and throughout the whole processing plant, indicating the presence of indigenous specific microbial communities in each processing compartment. The microbiota of drain biofilms was largely different from the microbiota of the drain water. A sampling approach based on drain water alone may thus only provide reliable information on planktonic bacterial cells but might not allow conclusions on the bacterial composition of the microbiota in biofilms. Copyright © 2016. Published by Elsevier B.V.

Keywords Pubmed: Bacteriaclassificationgeneticsisolation & purification
Environmental Microbiology
Floors and Floorcoveringsstandards
Food Handlingstandards
Listeria monocytogenes
RNA, Ribosomal, 16Sgenetics
Waste Watermicrobiology

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